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LIG3 Protein (AA 1-1015) (Strep Tag)

Crystallography grade LIG3 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
产品编号 ABIN3134693
发货至: 中国
  • 抗原 See all LIG3 蛋白
    LIG3 (Ligase III, DNA, ATP-Dependent (LIG3))
    蛋白类型
    Recombinant
    产品特性
    AA 1-1015
    宿主
    小鼠
    资源
    • 2
    • 1
    • 1
    Tobacco (Nicotiana tabacum)
    标记
    This LIG3 protein is labelled with Strep Tag.
    应用范围
    ELISA, Western Blotting (WB), SDS-PAGE (SDS)
    序列
    MTLAFKILFP RNLCALGRKE LCLFPEQNRW AAISQFSQWS ETNLLGGCCL LQRRKPVLAL QRGHLRPRAT HLTFWPGSHV GLCTGPCAMA EQRFCVDYAK RGTAGCKKCK EKIVKGVCRI GKVVPNPFSE SGGDMKEWYH IKCMFEKLER ARATTKKIED LTELEGWEEL EDNEKEQISQ HIADLSSKAA ATPKKKAAVQ AKLTTTGQVT SPVKGASFIT STNPRKFSGF SAAKPNNSEQ APSSPAPGTS LSASKCDPKH KDCLLREFRK LCAMVAENPS YNTKTQIIHD FLQKGSTGGF SDGFHGDVYL TVKLLLPGVI KSVYNLNDKQ IVKLFSRIFN CNPDDMARDL EQGDVSETIR IFFEQSKSFP PAAKSLLTIQ EVDAFLLHLS KLTKEDEQQQ ALQDIASRCT ANDLKCIIRL IKHDLKMNSG AKHVLDALDP NAYEAFKASR NLQDVVERVL HNEQEVEKDP GRRRALRVQA SLMTPVQPML AEACKSIEYA MKKCPNGMFS EIKYDGERVQ VHKKGDHFSY FSRSLKPVLP HKVAHFKDYI PKAFPGGQSM ILDSEVLLID NNTGKPLPFG TLGVHKKAAF QDANVCLFVF DCIYFNDVSL MDRPLCERRK FLHDNMVEIR NRIMFSEMKQ VTKASDLADM INRVIREGLE GLVLKDVKGT YEPGKRHWLK VKKDYLNEGA MADTADLVVL GAFYGQGSKG GMMSIFLMGC YDPDSQKWCT VTKCAGGHDD ATLARLQKEL VMVKISKDPS KIPSWLKINK IYYPDFIVPD PKKAAVWEIT GAEFSRSEAH TADGISIRFP RCTRIRDDKD WKSATNLPQL KELYQLSKDK ADFAVVAGDE ASPTTGGSSG ENEGTAGSAG PCKGPPSKSS ASAKTTEQKL NSPSSRGGIK PIPKHSPMKP GEKLAVKSSP VKVGMKRKAT DETPCLKKVL LDVFTGVRLY LPPSTPDFKR LKRYFVAFDG DLVQEFDMGS ATHVLGNREK NTDAQLVSSE WIWACIRKRR LIAPC
    Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
    产品特性
    Key Benefits:
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
    • These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    Expression System:

    • ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    • During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Concentration:
    • The concentration of our recombinant proteins is measured using the absorbance at 280nm.
    • The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
    • We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    纯化方法
    Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
    1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    纯度
    ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    内毒素水平
    Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
    质量等级
    Crystallography grade
    Top Product
    Discover our top product LIG3 蛋白
  • 应用备注
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    说明

    ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
    注意事项
    Avoid repeated freeze-thaw cycles.
    储存条件
    -80 °C
    储存方法
    Store at -80°C.
    有效期
    Unlimited (if stored properly)
  • 抗原
    LIG3 (Ligase III, DNA, ATP-Dependent (LIG3))
    别名
    Lig3 (LIG3 产品)
    别名
    orf36 Protein, DDBDRAFT_0218523 Protein, DDBDRAFT_0232266 Protein, DDB_0218523 Protein, DDB_0232266 Protein, LIG3 Protein, wu:fb12c02 Protein, wu:fd10b03 Protein, wu:fm79b09 Protein, LIG2 Protein, D11Wsu78e Protein, An02g06890 Protein, AO090003000668 Protein, lig3 Protein, DNA ligase III Protein, BRCT domain-containing protein Protein, DNA ligase 3 Protein, ligase III, DNA, ATP-dependent Protein, ligase III, DNA, ATP-dependent L homeolog Protein, orf36 Protein, lig3 Protein, LIG3 Protein, Lig3 Protein, Bm1_22840 Protein, ANI_1_2644024 Protein, AOR_1_1174154 Protein, LOC100634887 Protein, lig3.L Protein
    背景
    DNA ligase 3 (EC 6.5.1.1) (DNA ligase III) (Polydeoxyribonucleotide synthase [ATP] 3),FUNCTION: The alpha isoform interacts with DNA-repair protein XRCC1 and can correct defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents. The beta isoform does not interact with XRCC1 and may be specifically involved in the completion of homologous recombination events that occur during meiotic prophase.
    分子量
    113.1 kDa
    UniProt
    P97386
    途径
    DNA Damage Repair
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