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NEDD1 Protein (AA 1-660) (His tag)

Crystallography grade NEDD1 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
产品编号 ABIN3133765
发货至: 中国
  • 抗原 See all NEDD1 蛋白
    NEDD1 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 1 (NEDD1))
    蛋白类型
    Recombinant
    产品特性
    AA 1-660
    宿主
    小鼠
    资源
    • 1
    • 1
    Insect Cells
    标记
    This NEDD1 protein is labelled with His tag.
    应用范围
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    序列
    MQENLRFASS GDDVKIWDAS FLTLVDKFNP HTSPHGISSI CWSSNNNFLV TASSSGDKIV VSSCKCKPVP LLELAEGQKQ TCVDLNSTSM YLASGGLNNT VNIWDLKSKR LHRSLKDHKC EVTCVAYNWN DCYIASGSLS GEIILHSVTT NTSSTPFGHG SKQPIRHIKY SLFRKSLLGS VSDNGVVTLW DVNSQSSYHT FDSTHKAPAS GICFSPVNEL LFVTIGLDKR IILYDTSSKK LVKTLVADTP LTAVDFMPDG ATLAIGSSRG KIYQYDLRML KSPVKTISAH KTSVQCIAFQ YSTSLTKASL SKGSSNKATA VNKRSVPVSS SSGAAQNSGI VREAPSPSIA TVLPQPVTTA LGKGSGAAQD EAGLARSKST DIFSKETDAG KSQDFSSFDD TGKNSLGDMF SPIRDDAVVS KGGDESIGKG DGLDFLPQLN SVFPLRKNAG ASSSLVLHSS PLNVLMGSSG KEENESHEPS AESKRAYLGK QEPKDAMKQF TKLISSGAEP GILNTCPSSN QARNLEKFEK PEKDIEAQLI HEPSVNGSST TVPKAASSVT AGVASSLSEK IVDTLGNSRP GAPLTSVQIR FIQNMIQETL DDFREACHRD IVNLQVEMIK QFHIQLNEMH SLLERYSVNE GLVAEIERLR EENKRLRAHF
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    产品特性
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Nedd1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    纯化方法
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    纯度
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    过滤
    0.22 μm filtered
    内毒素水平
    Protein is endotoxin free.
    质量等级
    Crystallography grade
    Top Product
    Discover our top product NEDD1 蛋白
  • 应用备注
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    说明

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    注意事项
    Avoid repeated freeze-thaw cycles.
    储存条件
    -80 °C
    储存方法
    Store at -80°C.
    有效期
    Unlimited (if stored properly)
  • 抗原
    NEDD1 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 1 (NEDD1))
    别名
    Nedd1 (NEDD1 产品)
    别名
    MGC81767 Protein, MGC145237 Protein, GCP-WD Protein, TUBGCP7 Protein, neural precursor cell expressed, developmentally down-regulated 1 L homeolog Protein, neural precursor cell expressed, developmentally down-regulated 1 Protein, cilia and flagella associated protein 54 Protein, neural precursor cell expressed, developmentally down-regulated gene 1 Protein, nedd1.L Protein, nedd1 Protein, CFAP54 Protein, NEDD1 Protein, Nedd1 Protein
    背景
    Required for mitosis progression. Promotes the nucleation of microtubules from the spindle (By similarity). May play an important role during the embryonic development and differentiation of the central nervous system. {ECO:0000250}.
    分子量
    72.2 kDa Including tag.
    UniProt
    P33215
    途径
    M Phase
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