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Cytokeratin 19 Protein (AA 1-403) (Strep Tag)

Crystallography grade KRT19 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
产品编号 ABIN3133209
发货至: 中国
  • 抗原 See all Cytokeratin 19 (KRT19) 蛋白
    Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))
    蛋白类型
    Recombinant
    产品特性
    AA 1-403
    宿主
    • 8
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    小鼠
    资源
    • 8
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    Tobacco (Nicotiana tabacum)
    标记
    This Cytokeratin 19 protein is labelled with Strep Tag.
    应用范围
    SDS-PAGE (SDS), Western Blotting (WB), ELISA
    序列
    MTSYSYRQTS AMSSFGGTGG GSVRIGSGGV FRAPSIHGGS GGRGVSVSST RFVTSSSGSY GGVRGGSFSG TLAVSDGLLS GNEKITMQNL NDRLASYLDK VRALEQANGE LEVKIRDWYQ KQGPGPSRDY NHYFKTIEDL RDKILGATID NSKIVLQIDN ARLAADDFRT KFETEHALRL SVEADINGLR RVLDELTLAR TDLEMQIESL KEELAYLKKN HEEEITALRS QVGGQVSVEV DSTPGVDLAK ILSEMRSQYE IMAEKNRKDA EATYLARIEE LNTQVAVHSE QIQISKTEVT DLRRTLQGLE IELQSQLSMK AALEGTLAET EARYGVQLSQ IQSVISGFEA QLSDVRADIE RQNQEYKQLM DIKSRLEQEI ATYRSLLEGQ EAHYNNLPTP KAI
    Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
    产品特性
    Key Benefits:
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
    • These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    Expression System:

    • ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    • During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Concentration:
    • The concentration of our recombinant proteins is measured using the absorbance at 280nm.
    • The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
    • We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    纯化方法
    Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
    1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    纯度
    ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    内毒素水平
    Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
    质量等级
    Crystallography grade
    Top Product
    Discover our top product KRT19 蛋白
  • 应用备注
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    说明

    ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
    注意事项
    Avoid repeated freeze-thaw cycles.
    储存条件
    -80 °C
    储存方法
    Store at -80°C.
    有效期
    Unlimited (if stored properly)
  • 抗原
    Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))
    别名
    Krt19 (KRT19 产品)
    别名
    CK19 Protein, K19 Protein, K1CS Protein, AI663979 Protein, EndoC Protein, Krt-1.19 Protein, Krt1-19 Protein, Ka19 Protein, k19 Protein, ck19 Protein, k1cs Protein, krt9 Protein, krt15 Protein, MGC76282 Protein, GK-19 Protein, MGC83069 Protein, KRT19 Protein, keratin 19 Protein, keratin 19 L homeolog Protein, keratin, type I cytoskeletal 19 Protein, KRT19 Protein, Krt19 Protein, krt19 Protein, krt19.L Protein, LOC100344434 Protein, LOC101117946 Protein
    背景
    Keratin, type I cytoskeletal 19 (Cytokeratin-19) (CK-19) (Keratin-19) (K19),FUNCTION: Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle (By similarity). {ECO:0000250}.
    分子量
    44.5 kDa
    UniProt
    P19001
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