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PIM1 Protein (AA 1-397) (His tag)

Crystallography grade PIM1 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
产品编号 ABIN3132574
发货至: 中国
  • 抗原 See all PIM1 蛋白
    PIM1 (Pim-1 Oncogene (PIM1))
    蛋白类型
    Recombinant
    产品特性
    AA 1-397
    宿主
    • 9
    • 3
    • 1
    • 1
    • 1
    小鼠
    资源
    • 5
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Insect Cells
    标记
    This PIM1 protein is labelled with His tag.
    应用范围
    SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)
    序列
    MGPAAPLALP PPALPDPAGE PARGQPRQRP QSSSDSPSAL RASRSQSRNA TRSLSPGRRL SPSSLRRRCC SSRHRRRTDT LEVGMLLSKI NSLAHLRARP CNDLHATKLA PGKEKEPLES QYQVGPLLGS GGFGSVYSGI RVADNLPVAI KHVEKDRISD WGELPNGTRV PMEVVLLKKV SSDFSGVIRL LDWFERPDSF VLILERPEPV QDLFDFITER GALQEDLARG FFWQVLEAVR HCHNCGVLHR DIKDENILID LSRGEIKLID FGSGALLKDT VYTDFDGTRV YSPPEWIRYH RYHGRSAAVW SLGILLYDMV CGDIPFEHDE EIIKGQVFFR QTVSSECQHL IKWCLSLRPS DRPSFEEIRN HPWMQGDLLP QAASEIHLHS LSPGSSK
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    产品特性
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Pim1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    纯化方法
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    纯度
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    过滤
    0.22 μm filtered
    内毒素水平
    Protein is endotoxin free.
    质量等级
    Crystallography grade
    Top Product
    Discover our top product PIM1 蛋白
  • 应用备注
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    说明

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    注意事项
    Avoid repeated freeze-thaw cycles.
    储存条件
    -80 °C
    储存方法
    Store at -80°C.
    有效期
    Unlimited (if stored properly)
  • 抗原
    PIM1 (Pim-1 Oncogene (PIM1))
    别名
    Pim1 (PIM1 产品)
    别名
    PIM Protein, Pim-1 Protein, PIM1 Protein, pim Protein, pim-1 Protein, pim3 Protein, Pim-1 proto-oncogene, serine/threonine kinase Protein, proviral integration site 1 Protein, Pim-1 proto-oncogene, serine/threonine kinase L homeolog Protein, PIM1 Protein, Pim1 Protein, pim1 Protein, pim1.L Protein
    背景
    Proto-oncogene with serine/threonine kinase activity involved in cell survival and cell proliferation and thus providing a selective advantage in tumorigenesis. Exerts its oncogenic activity through: the regulation of MYC transcriptional activity, the regulation of cell cycle progression and by phosphorylation and inhibition of proapoptotic proteins (BAD, MAP3K5, FOXO3). Phosphorylation of MYC leads to an increase of MYC protein stability and thereby an increase of transcriptional activity. The stabilization of MYC exerted by PIM1 might explain partly the strong synergism between these two oncogenes in tumorigenesis. Mediates survival signaling through phosphorylation of BAD, which induces release of the anti-apoptotic protein Bcl-X(L)/BCL2L1. Phosphorylation of MAP3K5, an other proapoptotic protein, by PIM1, significantly decreases MAP3K5 kinase activity and inhibits MAP3K5-mediated phosphorylation of JNK and JNK/p38MAPK subsequently reducing caspase-3 activation and cell apoptosis. Stimulates cell cycle progression at the G1-S and G2-M transitions by phosphorylation of CDC25A and CDC25C. Phosphorylation of CDKN1A, a regulator of cell cycle progression at G1, results in the relocation of CDKN1A to the cytoplasm and enhanced CDKN1A protein stability. Promote cell cycle progression and tumorigenesis by down-regulating expression of a regulator of cell cycle progression, CDKN1B, at both transcriptional and post-translational levels. Phosphorylation of CDKN1B,induces 14-3-3 binding, nuclear export and proteasome-dependent degradation. May affect the structure or silencing of chromatin by phosphorylating HP1 gamma/CBX3. Acts also as a regulator of homing and migration of bone marrow cells involving functional interaction with the CXCL12-CXCR4 signaling axis (By similarity). {ECO:0000250, ECO:0000269|PubMed:15199164, ECO:0000269|PubMed:15280015, ECO:0000269|PubMed:1825810, ECO:0000269|PubMed:18438430, ECO:0000269|PubMed:19687226}.
    分子量
    45.5 kDa Including tag.
    UniProt
    P06803
    途径
    Glycosaminoglycan Metabolic Process
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