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ROBO1 Protein (AA 20-858) (His tag)

Crystallography grade ROBO1 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
产品编号 ABIN3132299
发货至: 中国
  • 抗原 See all ROBO1 蛋白
    ROBO1 (Roundabout, Axon Guidance Receptor, Homolog 1 (ROBO1))
    蛋白类型
    Recombinant
    产品特性
    AA 20-858
    宿主
    • 9
    • 2
    • 1
    小鼠
    资源
    • 5
    • 5
    • 1
    • 1
    Insect Cells
    标记
    This ROBO1 protein is labelled with His tag.
    应用范围
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    序列
    SRLRQEDFPP RIVEHPSDLI VSKGEPATLN CKAEGRPTPT IEWYKGGERV ETDKDDPRSH RMLLPSGSLF FLRIVHGRKS RPDEGVYICV ARNYLGEAVS HNASLEVAIL RDDFRQNPSD VMVAVGEPAV MECQPPRGHP EPTISWKKDG SPLDDKDERI TIRGGKLMIT YTRKSDAGKY VCVGTNMVGE RESEVAELTV LERPSFVKRP SNLAVTVDDS AEFKCEARGD PVPTVRWRKD DGELPKSRYE IRDDHTLKIR KVTAGDMGSY TCVAENMVGK AEASATLTVQ EPPHFVVKPR DQVVALGRTV TFQCEATGNP QPAIFWRREG SQNLLFSYQP PQSSSRFSVS QTGDLTITNV QRSDVGYYIC QTLNVAGSII TKAYLEVTDV IADRPPPVIR QGPVNQTVAV DGTLILSCVA TGSPAPTILW RKDGVLVSTQ DSRIKQLESG VLQIRYAKLG DTGRYTCTAS TPSGEATWSA YIEVQEFGVP VQPPRPTDPN LIPSAPSKPE VTDVSKNTVT LSWQPNLNSG ATPTSYIIEA FSHASGSSWQ TAAENVKTET FAIKGLKPNA IYLFLVRAAN AYGISDPSQI SDPVKTQDVP PTSQGVDHKQ VQRELGNVVL HLHNPTILSS SSVEVHWTVD QQSQYIQGYK ILYRPSGASH GESEWLVFEV RTPTKNSVVI PDLRKGVNYE IKARPFFNEF QGADSEIKFA KTLEEAPSAP PRSVTVSKND GNGTAILVTW QPPPEDTQNG MVQEYKVWCL GNETKYHINK TVDGSTFSVV IPSLVPGIRY SVEVAASTGA GPGVKSEPQF IQLDSHGNPV SPEDQVSLAQ QISDVVRQP
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    产品特性
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Robo1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    纯化方法
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    纯度
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    过滤
    0.22 μm filtered
    内毒素水平
    Protein is endotoxin free.
    质量等级
    Crystallography grade
    Top Product
    Discover our top product ROBO1 蛋白
  • 应用备注
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    说明

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    注意事项
    Avoid repeated freeze-thaw cycles.
    储存条件
    -80 °C
    储存方法
    Store at -80°C.
    有效期
    Unlimited (if stored properly)
  • 抗原
    ROBO1 (Roundabout, Axon Guidance Receptor, Homolog 1 (ROBO1))
    别名
    Robo1 (ROBO1 产品)
    别名
    DUTT1 Protein, SAX3 Protein, AW494633 Protein, AW742721 Protein, Gm310 Protein, ROBO1 Protein, dutt1 Protein, roundabout Protein, sax3 Protein, MGC122264 Protein, roundabout-1 Protein, roundabout guidance receptor 1 Protein, roundabout, axon guidance receptor, homolog 1 (Drosophila) Protein, roundabout guidance receptor 1 L homeolog Protein, ROBO1 Protein, Robo1 Protein, robo1 Protein, robo1.L Protein
    背景
    Receptor for SLIT1 and SLIT2 that mediates cellular responses to molecular guidance cues in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development (PubMed:10433822, PubMed:24560577). Interaction with the intracellular domain of FLRT3 mediates axon attraction towards cells expressing NTN1 (PubMed:24560577). In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex (By similarity). Plays a role in the regulation of cell migration via its interaction with MYO9B, inhibits MYO9B-mediated stimulation of RHOA GTPase activity, and thereby leads to increased levels of active, GTP-bound RHOA (By similarity). May be required for lung development (PubMed:11734623). {ECO:0000250|UniProtKB:Q9Y6N7, ECO:0000269|PubMed:10433822, ECO:0000269|PubMed:11734623, ECO:0000269|PubMed:15091338, ECO:0000269|PubMed:24560577}.
    分子量
    93.1 kDa Including tag.
    UniProt
    O89026
    途径
    Positive Regulation of Endopeptidase Activity
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