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BHLHE40 Protein (AA 1-411) (His tag)

Crystallography grade BHLHE40 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
产品编号 ABIN3131464
发货至: 中国
  • 抗原 See all BHLHE40 蛋白
    BHLHE40 (Basic Helix-Loop-Helix Family, Member E40 (BHLHE40))
    蛋白类型
    Recombinant
    产品特性
    AA 1-411
    宿主
    • 3
    • 1
    • 1
    • 1
    • 1
    小鼠
    资源
    • 4
    • 1
    • 1
    • 1
    Insect Cells
    标记
    This BHLHE40 protein is labelled with His tag.
    应用范围
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    序列
    MERIPSAQPP PTCLPKAPGL EHGDLSGMDF AHMYQVYKSR RGIKRSEDSK ETYKLPHRLI EKKRRDRINE CIAQLKDLLP EHLKLTTLGH LEKAVVLELT LKHVKALTNL IDQQQQKIIA LQSGLQAGDL SGRNLEAGQE MFCSGFQTCA REVLQYLAKH ENTRDLKSSQ LVTHLHRVVS ELLQGGASRK PLDSAPKAVD LKEKPSFLAK GSEGPGKNCV PVIQRTFAPS GGEQSGSDTD TDSGYGGELE KGDLRSEQPY FKSDHGRRFA VGERVSTIKQ ESEEPPTKKS RMQLSEEEGH FAGSDLMGSP FLGPHPHQPP FCLPFYLIPP SATAYLPMLE KCWYPTSVPV LYPGLNTSAA ALSSFMNPDK IPTPLLLPQR LPSPLAHSSL DSSALLQALK QIPPLNLETK D
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    产品特性
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Bhlhe40 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    纯化方法
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    纯度
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    过滤
    0.22 μm filtered
    内毒素水平
    Protein is endotoxin free.
    质量等级
    Crystallography grade
    Top Product
    Discover our top product BHLHE40 蛋白
  • 应用备注
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    说明

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    注意事项
    Avoid repeated freeze-thaw cycles.
    储存条件
    -80 °C
    储存方法
    Store at -80°C.
    有效期
    Unlimited (if stored properly)
  • 抗原
    BHLHE40 (Basic Helix-Loop-Helix Family, Member E40 (BHLHE40))
    别名
    Bhlhe40 (BHLHE40 产品)
    别名
    BHLHB2 Protein, DEC1 Protein, HLHB2 Protein, SHARP-2 Protein, STRA13 Protein, Stra14 Protein, bhlhb2 Protein, zgc:76913 Protein, wu:fc28a06 Protein, wu:fm54h07 Protein, dec1 Protein, Bhlhb2 Protein, C130042M06Rik Protein, CR8 Protein, Clast5 Protein, Dec1 Protein, Sharp2 Protein, Stra13 Protein, basic helix-loop-helix family member e40 Protein, basic helix-loop-helix family, member e40 Protein, basic helix-loop-helix family member e40 L homeolog Protein, BHLHE40 Protein, bhlhe40 Protein, bhlhe40.L Protein, Bhlhe40 Protein
    背景
    Transcriptional repressor involved in the regulation of the circadian rhythm by negatively regulating the activity of the clock genes and clock-controlled genes. Acts as the negative limb of a novel autoregulatory feedback loop (DEC loop) which differs from the one formed by the PER and CRY transcriptional repressors (PER/CRY loop). Both these loops are interlocked as it represses the expression of PER1/2 and in turn is repressed by PER1/2 and CRY1/2. Represses the activity of the circadian transcriptional activator: CLOCK-ARNTL/BMAL1|ARNTL2/BMAL2 heterodimer by competing for the binding to E-box elements (5'-CACGTG-3') found within the promoters of its target genes. Negatively regulates its own expression and the expression of DBP and BHLHE41/DEC2. Acts as a corepressor of RXR and the RXR-LXR heterodimers and represses the ligand-induced RXRA and NR1H3/LXRA transactivation activity. May function as a transcriptional factor for neuronal differentiation. {ECO:0000269|PubMed:14672706, ECO:0000269|PubMed:18411297, ECO:0000269|PubMed:19786558}.
    分子量
    46.3 kDa Including tag.
    UniProt
    O35185
    途径
    Photoperiodism
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