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MTTP Protein (AA 22-894) (His tag)

Crystallography grade MTTP 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
产品编号 ABIN3131257
发货至: 中国
  • 抗原 See all MTTP 蛋白
    MTTP (Microsomal Triglyceride Transfer Protein (MTTP))
    蛋白类型
    Recombinant
    产品特性
    AA 22-894
    宿主
    • 3
    • 1
    小鼠
    资源
    • 2
    • 1
    • 1
    Insect Cells
    标记
    This MTTP protein is labelled with His tag.
    应用范围
    Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
    序列
    HTTGLSLNNE RLYKLTYSTE VFLDGGKGKP QDSVGYKISS DVDVVLLWRN PDGDDDQVIQ VTITAVNVEN AGQQRGEKSI FQGKSTPKII GKDNLEALQR PMLLHLVRGK VKEFYSYENE PVGIENLKRG LASLFQMQLS SGTTNEVDIS GDCKVTYQAQ QDKVVKIKAL DTCKIERSGF TTANQVLGVS SKATSVTTYK IEDSFVTAVL AEETRAFALN FQQTIAGKIV SKQKLELKTT EAGPRMIPGK QVAGVIKAVD SKYKAIPIVG QVLERVCKGC PSLAEHWKSI RKNLEPENLS KAEAVQSFLA FIQHLRTSRR EEILQILKAE KKEVLPQLVD AVTSAQTPDS LEAILDFLDF KSDSSIILQE RFLYACGFAT HPDEELLRAL LSKFKGSFAS NDIRESVMII IGALVRKLCQ NEGCKLKAVV EAKKLILGGL EKPEKKEDTT MYLLALKNAL LPEGIPLLLK YAEAGEGPVS HLATTVLQRY DVSFITDEVK KTLNRIYHQN RKVHEKTVRT TAAAVILKNP SYMDVKNILL SIGELPKEMN KYMLTVVQDI LHFEMPASKM IRRVLKEMAV HNYDRFSKSG SSSAYTGYVE RSPRAASTYS LDILYSGSGI LRRSNLNIFQ YIGKAELHGS QVVIEAQGLE GLIAATPDEG EENLDSYAGM SAILFDVQLR PVTFFNGYSD LMSKMLSASG DPVSVVKGLI LLIDHSQDIQ LQSGLKANME IQGGLAIDIS GSMEFSLWYR ESKTRVKNRV AVVITSDVTV DASFVKAGVE SRAETEAGLE FISTVQFSQY PFLVCMQMDK AEAPLRQFET KYERLSTGRG YVSRRRKESL VAGCELPLHQ ENSEMCNVVF PPQPESDNSG GWF
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    产品特性
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Mttp Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    纯化方法
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    纯度
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    过滤
    0.22 μm filtered
    内毒素水平
    Protein is endotoxin free.
    质量等级
    Crystallography grade
    Top Product
    Discover our top product MTTP 蛋白
  • 应用备注
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    说明

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    注意事项
    Avoid repeated freeze-thaw cycles.
    储存条件
    -80 °C
    储存方法
    Store at -80°C.
    有效期
    Unlimited (if stored properly)
  • 抗原
    MTTP (Microsomal Triglyceride Transfer Protein (MTTP))
    别名
    Mttp (MTTP 产品)
    别名
    ABL Protein, MTP Protein, 1810043K16Rik Protein, CG9342 Protein, CT3751 Protein, Dmel\\CG9342 Protein, dMTP Protein, wu:fd36b01 Protein, zgc:111876 Protein, microsomal triglyceride transfer protein Protein, Microsomal triacylglycerol transfer protein Protein, MTTP Protein, Mttp Protein, Mtp Protein, mttp Protein
    背景
    Catalyzes the transport of triglyceride, cholesteryl ester, and phospholipid between phospholipid surfaces. Required for the secretion of plasma lipoproteins that contain apolipoprotein B. May be involved in regulating cholesteryl ester biosynthesis in cells that produce lipoproteins. Loads phospholipid into the C1D1 antigen-binding groove. Isoform 2 is critical for the development of natural killer T (NKT) cells. May have a role in the biogenesis of lipid droplets. {ECO:0000269|PubMed:17312007, ECO:0000269|PubMed:17635917, ECO:0000269|PubMed:18502767}.
    分子量
    97.8 kDa Including tag.
    UniProt
    O08601
    途径
    Transition Metal Ion Homeostasis
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