电话:
400-7060-959
传真:
+86 10 56315212-8813
电子邮件:
orders@antibodies-online.cn

RAP1GAP Protein (AA 1-663) (Strep Tag)

Crystallography grade RAP1GAP 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
产品编号 ABIN3130783
发货至: 中国
  • 抗原 See all RAP1GAP 蛋白
    RAP1GAP (RAP1 GTPase Activating Protein (RAP1GAP))
    蛋白类型
    Recombinant
    产品特性
    AA 1-663
    宿主
    小鼠
    资源
    • 1
    • 1
    • 1
    Tobacco (Nicotiana tabacum)
    标记
    This RAP1GAP protein is labelled with Strep Tag.
    应用范围
    ELISA, Western Blotting (WB), SDS-PAGE (SDS)
    序列
    MIEKMQGSRM DEQRCSFPPP LKTEEDYIPY PSVHEVLGRE GPFPLILLPQ FGGYWIEGTN HEISSLPETE PLQSPTTKVK LECNPTARIY RKHFLGKEHF NYYSLDTALG HLVFSLKYDV IGDQEHLRLL LRTKCRTHHD VIPISCLTEF PNVVQMAKLV CEDVNVDRFY PVLYPKASRL IVTFDEHVIS NNFKFGVIYQ KLGQTSEEEL FSTNEESPAF VEFLEFLGQK VKLQDFKGFR GGLDVTHGQT GTESVYCNFR NKEIMFHVST KLPYTEGDAQ QLQRKRHIGN DIVAVVFQDE NTPFVPDMIA SNFLHAYVVV QAEGGGPDGP LYKVSVTARD DVPFFGPPLP DPAVFRKGPE FQEFLLTKLI NAEYACYKAE KFAKLEERTR AALLETLYEE LHIHSQSMMG LGGDDDKMEN GSGGGGFFES FKRVIRSRSQ SMDAMGLSNK KPNTVSTSHS GSFTPNNPDL AKAAGISLIV PGKSPTRKKS GPFGSRRSSA IGIENIQEVQ EKRESPPAGQ KTPDSGHVSQ EPKSENSSTQ SSPEMPTTKN RVESAAQRTE VLQGFSRSSS SASSFTSVVE ETEGVDGDDT GLESVSSSGT PHKRDSFLYS TWLDDSVSTT SGGSSPGLTR SPHPDVGKSG DPACPEIKIQ LETSEQHTPQ MGC
    Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
    产品特性
    Key Benefits:
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
    • These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    Expression System:

    • ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    • During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Concentration:
    • The concentration of our recombinant proteins is measured using the absorbance at 280nm.
    • The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
    • We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    纯化方法
    Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
    1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    纯度
    ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    内毒素水平
    Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
    质量等级
    Crystallography grade
    Top Product
    Discover our top product RAP1GAP 蛋白
  • 应用备注
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    说明

    ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
    注意事项
    Avoid repeated freeze-thaw cycles.
    储存条件
    -80 °C
    储存方法
    Store at -80°C.
    有效期
    Unlimited (if stored properly)
  • 抗原
    RAP1GAP (RAP1 GTPase Activating Protein (RAP1GAP))
    别名
    Rap1gap (RAP1GAP 产品)
    别名
    RAP1GA1 Protein, RAP1GAP1 Protein, RAP1GAPII Protein, RAPGAP Protein, 1300019I11Rik Protein, 2310004O14Rik Protein, AI427470 Protein, Rap1ga1 Protein, RAP1 Protein, RAP1GAP Protein, rap1ga1 Protein, rap1gap1 Protein, rap1gapii Protein, rapgap Protein, zgc:175180 Protein, DKFZp459A114 Protein, RAP1 GTPase activating protein Protein, Rap1 GTPase-activating protein Protein, RAP1 GTPase activating protein 1 Protein, RAP1 GTPase activating protein L homeolog Protein, Rap1 GTPase-activating protein 1 Protein, si:dkey-166d12.2 Protein, RAP1GAP Protein, Rap1gap Protein, RAP1GAP1 Protein, rap1gap.L Protein, Tsp_09520 Protein, rap1gap Protein, si:dkey-166d12.2 Protein
    背景
    Rap1 GTPase-activating protein 1 (Rap1GAP) (Rap1GAP1) (ARPP-90),FUNCTION: GTPase activator for the nuclear Ras-related regulatory protein RAP-1A (KREV-1), converting it to the putatively inactive GDP-bound state. {ECO:0000250}.
    分子量
    73.4 kDa
    UniProt
    A2ALS5
You are here:
客服