PPIE Protein (AA 1-301) (Strep Tag)
-
- 抗原 See all PPIE 蛋白
- PPIE (Peptidylprolyl Isomerase E (Cyclophilin E) (PPIE))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-301
-
宿主
- 小鼠
-
资源
- Tobacco (Nicotiana tabacum)
- 标记
- This PPIE protein is labelled with Strep Tag.
- 应用范围
- Western Blotting (WB), SDS-PAGE (SDS), ELISA
- 序列
-
MATTKRVLYV GGLAEEVDDK VLHAAFIPFG DITDIQIPLD YETEKHRGFA FVEFELAEDA AAAIDNMNES ELFGRTIRVN LAKPMRIKEG SSRPVWSDDD WLKKFSGKTL EENKEEEGPE PPKAEAQEGE PTAKKARSNP QVYMDIKIGN KPAGRIQMLL RSDVVPMTAE NFRCLCTHEK GFGFKGSSFH RIIPQFMCQG GDFTNHNGTG GKSIYGKKFD DENFILKHTG PGLLSMANSG PNTNGSQFFL TCDKTDWLDG KHVVFGEVTE GLDVLRQIEA QGSKDGKPKQ KVMIADCGEY M
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - 产品特性
-
Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
-
Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 内毒素水平
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product PPIE 蛋白
-
Peptidylprolyl Isomerase E (Cyclophilin E) (PPIE) (AA 2-301) protein (His tag)
PPIE 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Peptidylprolyl Isomerase E (Cyclophilin E) (PPIE) (AA 1-301) protein (GST tag)PPIE 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Peptidylprolyl Isomerase E (Cyclophilin E) (PPIE) (AA 2-100) protein (GST tag)PPIE 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Peptidylprolyl Isomerase E (Cyclophilin E) (PPIE) (AA 1-301) (Active) protein (His tag)PPIE 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant SDS, Func Active
Peptidylprolyl Isomerase E (Cyclophilin E) (PPIE) (AA 1-301) protein (Strep Tag)Crystallography grade PPIE 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Peptidylprolyl Isomerase E (Cyclophilin E) (PPIE) (Transcript Variant 1) protein (His tag)PPIE 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
-
- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
-
ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - 限制
- 仅限研究用
-
- 状态
- Liquid
- 缓冲液
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
-
- 抗原
- PPIE (Peptidylprolyl Isomerase E (Cyclophilin E) (PPIE))
- 别名
- Ppie (PPIE 产品)
- 别名
- zgc:112471 Protein, CG4886 Protein, CYP33 Protein, Cyp33 Protein, Dcyp33 Protein, Dmel\\CG4886 Protein, PPIE Protein, cyp-33 Protein, cyp33 Protein, cype Protein, CYP-33 Protein, 2010010D16Rik Protein, Ab1-210 Protein, peptidylprolyl isomerase E (cyclophilin E) Protein, cyclophilin-33 Protein, peptidylprolyl isomerase E Protein, peptidylprolyl isomerase E (cyclophilin E) L homeolog Protein, ppie Protein, cyp33 Protein, PPIE Protein, ppie.L Protein, Ppie Protein
- 背景
- Peptidyl-prolyl cis-trans isomerase E (PPIase E) (EC 5.2.1.8) (Cyclophilin E) (Cyclophilin-33) (Rotamase E),FUNCTION: Involved in pre-mRNA splicing as component of the spliceosome. Combines RNA-binding and PPIase activities. Binds mRNA and has a preference for single-stranded RNA molecules with poly-A and poly-U stretches, suggesting it binds to the poly(A)-region in the 3'-UTR of mRNA molecules. Catalyzes the cis-trans isomerization of proline imidic peptide bonds in proteins. Inhibits KMT2A activity, this requires proline isomerase activity. {ECO:0000250|UniProtKB:Q9UNP9}.
- 分子量
- 33.4 kDa
- UniProt
- Q9QZH3
-
