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CBLL1 Protein (AA 1-491) (Strep Tag)

Crystallography grade CBLL1 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
产品编号 ABIN3130100
发货至: 中国
  • 抗原 See all CBLL1 蛋白
    CBLL1 (Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence-Like 1 (CBLL1))
    蛋白类型
    Recombinant
    产品特性
    AA 1-491
    宿主
    • 2
    • 1
    • 1
    小鼠
    资源
    • 2
    • 1
    • 1
    Tobacco (Nicotiana tabacum)
    标记
    This CBLL1 protein is labelled with Strep Tag.
    应用范围
    ELISA, Western Blotting (WB), SDS-PAGE (SDS)
    序列
    MDHTDNELQG TNSSGSLGGL DVRRRIPIKL ISKQASKVKP APRTQRTVSR MPAKAPQGDE EGFDYNEEQR YDCKGGELFG NQRRFPGHLF WDFKINILGE KDDTPVHFCD KCGLPIKVYG RMIPCKHVFC YDCAILHEKK GDKMCPGCSD PVQRIEQCTR GSLFMCSIVQ GCKRTYLSQR DLQAHINHRH MRAGKPVTRA SLENVHPPIA PPPTDIPDRF IMPPDKHHMS HIPPKQHIMM PPPPLQHVPH EHYNQPHEDI RAPPAELSMA PPPPRSVSQE TFRISTRKHS NLITVPIQDD SSSGAREPPP PAPAPAHHHP EYQGQPVVSH PHHIMPPQQH YAPPPPPPPP ISHPMPHPPQ AAGTPHLVYS QAPPPPMTSA PPPITPPPGH IIAQMPPYMN HPPPGPPPPQ HGGPPVTAPP PHHYNPNSLP QFTEDQGTLS PPFTQPGGMS PGIWPAPRGP PPPPRMQGPP SQTPLPGPHH PDQTRYRPYY Q
    Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
    产品特性
    Key Benefits:
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
    • These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    Expression System:

    • ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    • During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Concentration:
    • The concentration of our recombinant proteins is measured using the absorbance at 280nm.
    • The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
    • We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    纯化方法
    Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
    1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    纯度
    ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    内毒素水平
    Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
    质量等级
    Crystallography grade
    Top Product
    Discover our top product CBLL1 蛋白
  • 应用备注
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    说明

    ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
    注意事项
    Avoid repeated freeze-thaw cycles.
    储存条件
    -80 °C
    储存方法
    Store at -80°C.
    有效期
    Unlimited (if stored properly)
  • 抗原
    CBLL1 (Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence-Like 1 (CBLL1))
    别名
    Cbll1 (CBLL1 产品)
    别名
    HAKAI Protein, RNF188 Protein, zgc:55308 Protein, CBLL1 Protein, AI467391 Protein, Hakai Protein, c-Cbl-like Protein, cbll1 Protein, hakai Protein, rnf188 Protein, Cbl proto-oncogene like 1 Protein, Cbl proto-oncogene-like 1, E3 ubiquitin protein ligase S homeolog Protein, Cbl proto-oncogene-like 1, E3 ubiquitin protein ligase Protein, Casitas B-lineage lymphoma-like 1 Protein, Cbl proto-oncogene-like 1, E3 ubiquitin protein ligase L homeolog Protein, CBLL1 Protein, cbll1.S Protein, Cbll1 Protein, cbll1 Protein, cbll1.L Protein
    背景
    E3 ubiquitin-protein ligase Hakai (EC 2.3.2.27) (Casitas B-lineage lymphoma-transforming sequence-like protein 1) (c-Cbl-like protein 1) (E-cadherin binding protein E7) (RING-type E3 ubiquitin transferase Hakai),FUNCTION: E3 ubiquitin-protein ligase that mediates ubiquitination of several tyrosine-phosphorylated Src substrates, including CDH1, CTTN and DOK1 (PubMed:11836526, PubMed:22252131). Targets CDH1 for endocytosis and degradation (PubMed:11836526). Associated component of the WMM complex, a complex that mediates N6-methyladenosine (m6A) methylation of RNAs, a modification that plays a role in the efficiency of mRNA splicing and RNA processing (PubMed:29535189, PubMed:29547716). Its function in the WMM complex is unknown (PubMed:29535189, PubMed:29547716). {ECO:0000269|PubMed:11836526, ECO:0000269|PubMed:22252131, ECO:0000269|PubMed:29535189, ECO:0000269|PubMed:29547716}.
    分子量
    54.4 kDa
    UniProt
    Q9JIY2
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