SEC13 Protein (AA 2-322) (His tag)
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- 抗原 See all SEC13 蛋白
- SEC13 (SEC13 Homolog (SEC13))
- 蛋白类型
- Recombinant
- 产品特性
- AA 2-322
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宿主
- 小鼠
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资源
- Insect Cells
- 标记
- This SEC13 protein is labelled with His tag.
- 应用范围
- ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)
- 序列
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VSVMNTVDTS HEDMIHDAQM DYYGTRLATC SSDRSVKIFD VRNGGQILIA DLRGHEGPVW QVAWAHPMYG NILASCSYDR KVIIWKEENG TWEKTHEHSG HDSSVNSVCW APHDYGLILA CGSSDGAISL LTYTGEGQWE VKKINNAHTI GCNAVSWAPA VVPGSLIDQP SGQKPNYIKK FASGGCDNLI KLWREEEDGQ WKEEQKLEAH SDWVRDVAWA PSIGLPTSTI ASCSQDGRVF IWTCDDASGN MWSPKLLHKF NDVVWHVSWS ITANILAVSG GDNKVTLWKE SVDGQWVCIS DVNKGQGSVS ASITEGQQNE Q
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Sec13 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product SEC13 蛋白
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SEC13 Homolog (SEC13) (AA 1-325) protein (GST tag)
SEC13 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
SEC13 Homolog (SEC13) (AA 1-322) protein (GST tag)SEC13 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
SEC13 Homolog (SEC13) (AA 226-324) protein (GST tag)SEC13 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
SEC13 Homolog (SEC13) (AA 2-322) protein (His tag)Crystallography grade SEC13 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
SEC13 Homolog (SEC13) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)SEC13 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- SEC13 (SEC13 Homolog (SEC13))
- 别名
- Sec13 (SEC13 产品)
- 别名
- D3S1231E Protein, SEC13L1 Protein, SEC13R Protein, npp-20 Protein, 1110003H02Rik Protein, Sec13l1 Protein, Sec13r Protein, Protein SEC13 homolog Protein, SEC13 homolog, nuclear pore and COPII coat complex component Protein, npp-20 Protein, SEC13 Protein, Sec13 Protein
- 背景
- Functions as a component of the nuclear pore complex (NPC) and the COPII coat. At the endoplasmic reticulum, SEC13 is involved in the biogenesis of COPII-coated vesicles. {ECO:0000250|UniProtKB:P55735}., As a component of the GATOR subcomplex GATOR2, functions as an activator of the amino acid-sensing branch of the TORC1 pathway. Inhibits the GATOR1 subcomplex, an inhibitor of the amino acid-sensing branch of the TORC1 pathway. Functions downstream and is regulated by amino acid sensors like SESN2. {ECO:0000250|UniProtKB:P55735}.
- 分子量
- 36.4 kDa Including tag.
- UniProt
- Q9D1M0
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