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PPCS Protein (AA 2-311) (His tag)

Crystallography grade PPCS 宿主: 小鼠 宿主: 大肠杆菌(E. Coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB, Crys
产品编号 ABIN3126996
发货至: 中国
  • 抗原 See all PPCS 蛋白
    PPCS (Phosphopantothenoylcysteine Synthetase (PPCS))
    蛋白类型
    Recombinant
    产品特性
    AA 2-311
    宿主
    小鼠
    资源
    • 2
    大肠杆菌(E. Coli)
    标记
    This PPCS protein is labelled with His tag.
    应用范围
    SDS-PAGE (SDS), ELISA, Western Blotting (WB), Crystallization (Crys)
    序列
    AEMDLVAELP RPAGAARWAE VMARFAARLG EQGRRVVLIT SGGTKVPLEA RAVRFLDNFS SGRRGAASAE VFLAAGYGVL FLYRARSAFP YAHRFPPQAW LSALRPSGPA QSGKLSLEAE ENALPGFAAA LQSYQEAAAA GTFLAVEFTT LADYLHLLQA AALALSPLGS SAMFYLAAAV SDFYIPVSEM PEHKIHSSGG PLQITMKMVP KMLSPLVKDW APKAFVVSFK LETDPDIIIS RARNALEVYQ HQVVVANILE SIKSFVIIVT KDSETELLLS EEEVAKGLVI EEKIVDDLRS RHTAFICDKN
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    产品特性
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Ppcs Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    纯化方法
    Two step purification of proteins expressed in bacterial culture:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    纯度
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    过滤
    0.22 μm filtered
    内毒素水平
    Endotoxin has not been removed. Please contact us if you require endotoxin removal.
    质量等级
    Crystallography grade
    Top Product
    Discover our top product PPCS 蛋白
  • 应用备注
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    说明

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    注意事项
    Avoid repeated freeze-thaw cycles.
    储存条件
    -80 °C
    储存方法
    Store at -80°C.
    有效期
    Unlimited (if stored properly)
  • 抗原
    PPCS (Phosphopantothenoylcysteine Synthetase (PPCS))
    别名
    Ppcs (PPCS 产品)
    别名
    RGD1311399 Protein, BcDNA:GH03502 Protein, CG5629 Protein, Dmel\\CG5629 Protein, GH03502 Protein, PPCS Protein, anon-WO0118547.401 Protein, dPPCS Protein, GB14583 Protein, MGC80633 Protein, im:7147515 Protein, zgc:136566 Protein, DFP Protein, 6330579B17Rik Protein, AI413471 Protein, AI790434 Protein, phosphopantothenoylcysteine synthetase Protein, Phosphopantothenoylcysteine synthetase Protein, phosphopantothenate--cysteine ligase Protein, phosphopantothenoylcysteine synthetase L homeolog Protein, Ppcs Protein, LOC411581 Protein, ppcs.L Protein, PPCS Protein, ppcs Protein
    背景
    Catalyzes the first step in the biosynthesis of coenzyme A from vitamin B5, where cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine. {ECO:0000250}.
    分子量
    34.6 kDa Including tag.
    UniProt
    Q8VDG5
    途径
    Ribonucleoside Biosynthetic Process
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