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SEH1L Protein (AA 1-360) (His tag)

Crystallography grade SEH1L 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB, Crys
产品编号 ABIN3126693
发货至: 中国
  • 抗原 See all SEH1L 蛋白
    SEH1L (SEH1-Like (SEH1L))
    蛋白类型
    Recombinant
    产品特性
    AA 1-360
    宿主
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    小鼠
    资源
    • 7
    • 1
    • 1
    Insect Cells
    标记
    This SEH1L protein is labelled with His tag.
    应用范围
    ELISA, SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys)
    序列
    MFVARSIAAD HKDLIHDVSF DFHGRRMATC SSDQSVKVWD KSESGDWHCT ASWKTHSGSV WRVTWAHPEF GQVLASCSFD RTAAVWEEIV GESNDKLRGQ SHWVKRTTLV DSRTSVTDVK FAPKHMGLML ATCSADGIVR VYEAPDVMNL SQWSLQHEVS CKLCCSCISW NPSSSRAHPP MIAVGSDDSS PNSMAKVQIF EYNENTRKYA KAETLMTVTD PVHDIAFAPN LGRSFHILAV ATKDVRIFTL KPLRKELTSS GGPTKFEIHI VAQFDNHNSQ VWRVSWNITG TVLASSGDDG CVRLWKANYM DNWKCTGILK GNGSPVNGSS QLGNSNPSLS SNIPNLQNSL NGTSASRKHS
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    产品特性
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Seh1l Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    纯化方法
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    纯度
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    过滤
    0.22 μm filtered
    内毒素水平
    Protein is endotoxin free.
    质量等级
    Crystallography grade
    Top Product
    Discover our top product SEH1L 蛋白
  • 应用备注
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    说明

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    注意事项
    Avoid repeated freeze-thaw cycles.
    储存条件
    -80 °C
    储存方法
    Store at -80°C.
    有效期
    Unlimited (if stored properly)
  • 抗原
    SEH1L (SEH1-Like (SEH1L))
    别名
    Seh1l (SEH1L 产品)
    别名
    2610007A16Rik Protein, AW540070 Protein, SEC13L Protein, SEH1A Protein, SEH1B Protein, Seh1 Protein, seh1l-a Protein, fa02g03 Protein, wu:fa02g03 Protein, zgc:55977 Protein, SEH1-like (S. cerevisiae Protein, SEH1 like nucleoporin Protein, SEH1 like nucleoporin L homeolog Protein, SEH1-like (S. cerevisiae) Protein, Seh1l Protein, SEH1L Protein, seh1l.L Protein, seh1l Protein
    背景
    Component of the Nup107-160 subcomplex of the nuclear pore complex (NPC). The Nup107-160 subcomplex is required for the assembly of a functional NPC. The Nup107-160 subcomplex is also required for normal kinetochore microtubule attachment, mitotic progression and chromosome segregation. This subunit plays a role in recruitment of the Nup107-160 subcomplex to the kinetochore. {ECO:0000250|UniProtKB:Q96EE3}., As a component of the GATOR subcomplex GATOR2, functions as an activator of the amino acid-sensing branch of the TORC1 pathway. Inhibits the GATOR1 subcomplex, an inhibitor of the amino acid-sensing branch of the TORC1 pathway. Functions downstream and is regulated by amino acid sensors like SESN2. {ECO:0000250|UniProtKB:Q96EE3}.
    分子量
    40.7 kDa Including tag.
    UniProt
    Q8R2U0
    途径
    Maintenance of Protein Location
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