PABPN1 Protein (AA 2-302) (His tag)
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- 抗原 See all PABPN1 蛋白
- PABPN1 (Poly A Binding Protein Nuclear 1 (PABPN1))
- 蛋白类型
- Recombinant
- 产品特性
- AA 2-302
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宿主
- 小鼠
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资源
- Insect Cells
- 标记
- This PABPN1 protein is labelled with His tag.
- 应用范围
- ELISA, SDS-PAGE (SDS), Crystallization (Crys), Western Blotting (WB)
- 序列
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AAAAAAAAAA GAAGGRGSGP GRRRHLVPGA GGEAGEGDPG GAGDYGNGLE SEELEPGELL PEPEPEEEPP RPRAPPGAPG PGPGSGAPGS QEEEEEPGLV EADPGDGAIE DPELEAIKAR VREMEEEAEK LKELQNEVEK QMNMSPPPGN AGPVIMSLEE KMEADARSIY VGNVDYGATA EELEAHFHGC GSVNRVTILC DKFSGHPKGF AYIEFSDKES VRTSLALDES LFRGRQIKVI PKRTNRPGIS TTDRGFPRSR YRARTTNYNS SRSRFYSGFN SRPRGRIYRG RARATSWYSP Y
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Pabpn1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product PABPN1 蛋白
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Poly A Binding Protein Nuclear 1 (PABPN1) (AA 2-306) protein (His tag)
Crystallography grade PABPN1 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, Crys, WB
Poly A Binding Protein Nuclear 1 (PABPN1) (AA 119-306) protein (His tag)PABPN1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS - PAGE SDS
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- PABPN1 (Poly A Binding Protein Nuclear 1 (PABPN1))
- 别名
- Pabpn1 (PABPN1 产品)
- 别名
- OPMD Protein, PAB2 Protein, PABII Protein, PABP-2 Protein, PABP2 Protein, Pabp3 Protein, mPABII Protein, ARABIDOPSIS POLY(A) BINDING 2 Protein, ATPAB2 Protein, F28A23.130 Protein, F28A23_130 Protein, POLY(A) BINDING PROTEIN 2 Protein, POLY(A)-BINDING PROTEIN 2 Protein, poly(A) binding protein 2 Protein, opmd Protein, pab2 Protein, pabp2 Protein, pabpii Protein, pabpn1 Protein, Pabpn1 Protein, Poly(A)-binding protein 2 Protein, fb19c03 Protein, wu:fb17e03 Protein, wu:fb18c06 Protein, wu:fb19c03 Protein, zgc:85979 Protein, zgc:92452 Protein, poly(A) binding protein nuclear 1 Protein, poly(A) binding protein, nuclear 1 Protein, poly(A) binding protein 2 Protein, poly(A) binding protein, nuclear 1 S homeolog Protein, poly(A) binding protein, nuclear 1 L homeolog Protein, polyadenylate-binding protein 2 Protein, poly(A) binding protein Pab2 Protein, PABPN1 Protein, Pabpn1 Protein, PAB2 Protein, pabpn1.S Protein, pabpn1.L Protein, LOC100728725 Protein, pab2 Protein, pabpn1 Protein
- 背景
- Involved in the 3'-end formation of mRNA precursors (pre-mRNA) by the addition of a poly(A) tail of 200-250 nt to the upstream cleavage product. Stimulates poly(A) polymerase (PAPOLA) conferring processivity on the poly(A) tail elongation reaction and controls also the poly(A) tail length. Increases the affinity of poly(A) polymerase for RNA. Is also present at various stages of mRNA metabolism including nucleocytoplasmic trafficking and nonsense-mediated decay (NMD) of mRNA. Cooperates with SKIP to synergistically activate E-box-mediated transcription through MYOD1 and may regulate the expression of muscle-specific genes. Binds to poly(A) and to poly(G) with high affinity. May protect the poly(A) tail from degradation (By similarity). {ECO:0000250}.
- 分子量
- 33.1 kDa Including tag.
- UniProt
- Q8CCS6
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