CD300a Protein (CD300A) (AA 28-185) (His tag)
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- 抗原 See all CD300a (CD300A) 蛋白
- CD300a (CD300A)
- 蛋白类型
- Recombinant
- 产品特性
- AA 28-185
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宿主
- 小鼠
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资源
- 大肠杆菌（E. Coli）
- 标记
- This CD300a protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)
- 序列
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LHGPSTMSGS VGESLSVSCR YEEKFKTKDK YWCRVSLKIL CKDIVKTSSS EEARSGRVTI RDHPDNLTFT VTYESLTLED ADTYMCAVDI SLFDGSLGFD KYFKIELSVV PSEDPVSSPG PTLETPVVST SLPTKGPALG SNTEGHREHD YSQGLRLP
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Cd300a Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in bacterial culture:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Endotoxin has not been removed. Please contact us if you require endotoxin removal.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product CD300A 蛋白
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CD300a (CD300A) (AA 18-180) protein (Fc Tag)
CD300A 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD300a (CD300A) (AA 18-180) protein (His tag)CD300A 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD300a (CD300A) (AA 28-185) protein (Fc Tag)CD300A 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD300a (CD300A) (AA 28-185) protein (His tag)CD300A 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD300a (CD300A) (AA 1-299) protein (GST tag)CD300A 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
CD300a (CD300A) (AA 207-318) protein (His tag)Crystallography grade CD300A 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
CD300a (CD300A) (AA 18-180) protein (His tag)Crystallography grade CD300A 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
CD300a (CD300A) (AA 18-299) protein (rho-1D4 tag)Crystallography grade CD300A 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
CD300a (CD300A) (AA 28-318) protein (rho-1D4 tag)Crystallography grade CD300A 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- CD300a (CD300A)
- 别名
- Cd300a (CD300A 产品)
- 别名
- CD300L-B1 Protein, CD300A Protein, clm8 Protein, B230315M08Rik Protein, Clm8 Protein, LMIR1 Protein, MAIR-I Protein, MAIR-Ia Protein, MMAC8 Protein, Pigr4 Protein, mcpir1 Protein, CLM-8 Protein, CMRF-35-H9 Protein, CMRF-35H Protein, CMRF35-H Protein, CMRF35-H9 Protein, CMRF35H Protein, CMRF35H9 Protein, IGSF12 Protein, IRC1 Protein, IRC1/IRC2 Protein, IRC2 Protein, IRp60 Protein, CD300 molecule like family member g Protein, CD300a molecule Protein, CMRF35-like molecule 8 Protein, CD300A molecule Protein, Cd300a molecule Protein, CD300LG Protein, CD300A Protein, LOC483289 Protein, clm8 Protein, Cd300a Protein
- 背景
- Inhibitory receptor which may contribute to the down-regulation of cytolytic activity in natural killer (NK) cells, and to the down-regulation of mast cell degranulation. {ECO:0000269|PubMed:12874256, ECO:0000269|PubMed:12893283, ECO:0000269|PubMed:16339535}.
- 分子量
- 18.3 kDa Including tag.
- UniProt
- Q6SJQ0
- 途径
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
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