SMUG1 Protein (AA 1-279) (Strep Tag)
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- 抗原 See all SMUG1 蛋白
- SMUG1 (Single-Strand-Selective Monofunctional Uracil-DNA Glycosylase 1 (SMUG1))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-279
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宿主
- 小鼠
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资源
- Tobacco (Nicotiana tabacum)
- 标记
- This SMUG1 protein is labelled with Strep Tag.
- 应用范围
- SDS-PAGE (SDS), Western Blotting (WB), ELISA
- 序列
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MAASQTFPLG PTHEPASALM EPLPCTRSLA EGFLEEELRL NAELSQLQFP EPVGVIYNPV DYAWEPHRNY VTRYCQGPKE VLFLGMNPGP FGMAQTGVPF GEVNVVRDWL GVGGPVLTPP QEHPKRPVLG LECPQSEVSG ARFWGFFRTL CGQPQVFFRH CFVHNLCPLL FLAPSGRNLT PAELPAKQRE QLLSICDAAL CRQVQLLGVR LVVGVGRLAE QRARRALAGL TPEVQVEGLL HPSPRSAQAN KGWEAAARER LQELGLLPLL TDEGSARPT
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - 产品特性
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 内毒素水平
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product SMUG1 蛋白
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Single-Strand-Selective Monofunctional Uracil-DNA Glycosylase 1 (SMUG1) (AA 1-177) protein (GST tag)
SMUG1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Single-Strand-Selective Monofunctional Uracil-DNA Glycosylase 1 (SMUG1) (AA 2-78) protein (GST tag)SMUG1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Single-Strand-Selective Monofunctional Uracil-DNA Glycosylase 1 (SMUG1) (AA 1-270) protein (Strep Tag)Crystallography grade SMUG1 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Single-Strand-Selective Monofunctional Uracil-DNA Glycosylase 1 (SMUG1) (AA 1-177), (full length) protein (His-SUMO Tag)SMUG1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
Single-Strand-Selective Monofunctional Uracil-DNA Glycosylase 1 (SMUG1) protein (Myc-DYKDDDDK Tag)SMUG1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- SMUG1 (Single-Strand-Selective Monofunctional Uracil-DNA Glycosylase 1 (SMUG1))
- 别名
- Smug1 (SMUG1 产品)
- 别名
- SMUG1 Protein, xsmug1 Protein, 1200013B09Rik Protein, A930006H09Rik Protein, C85220 Protein, FDG Protein, HMUDG Protein, UNG3 Protein, single-strand-selective monofunctional uracil-DNA glycosylase 1 Protein, single-strand selective monofunctional uracil DNA glycosylase Protein, single-strand-selective monofunctional uracil-DNA glycosylase 1 L homeolog Protein, SMUG1 Protein, smug1 Protein, smuG1 Protein, smuG Protein, LOC5577584 Protein, CpipJ_CPIJ002767 Protein, Smug1 Protein, smug1.L Protein
- 背景
- Single-strand selective monofunctional uracil DNA glycosylase (EC 3.2.2.-),FUNCTION: Recognizes base lesions in the genome and initiates base excision DNA repair. Acts as a monofunctional DNA glycosylase specific for uracil (U) residues in DNA with a preference for single-stranded DNA substrates. The activity is greater toward mismatches (U/G) compared to matches (U/A). Excises uracil (U), 5-formyluracil (fU) and uracil derivatives bearing an oxidized group at C5 [5-hydroxyuracil (hoU) and 5-hydroxymethyluracil (hmU)] in ssDNA and dsDNA, but not analogous cytosine derivatives (5-hydroxycytosine and 5-formylcytosine), nor other oxidized bases. The activity is damage-specific and salt-dependent. The substrate preference is the following: ssDNA > dsDNA (G pair) = dsDNA (A pair) at low salt concentration, and dsDNA (G pair) > dsDNA (A pair) > ssDNA at high salt concentration. {ECO:0000250|UniProtKB:Q53HV7}.
- 分子量
- 30.7 kDa
- UniProt
- Q6P5C5
- 途径
- DNA Damage Repair
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