TUBA1A Protein (AA 1-451) (Strep Tag)
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- 抗原 See all TUBA1A (Tuba1a) 蛋白
- TUBA1A (Tuba1a) (Tubulin, alpha 1a (Tuba1a))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-451
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宿主
- 小鼠
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资源
- Tobacco (Nicotiana tabacum)
- 标记
- This TUBA1A protein is labelled with Strep Tag.
- 应用范围
- ELISA, Western Blotting (WB), SDS-PAGE (SDS)
- 序列
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MRECISIHVG QAGVQIGNAC WELYCLEHGI QPDGQMPSDK TIGGGDDSFN TFFSETGAGK HVPRAVFVDL EPTVIDEVRT GTYRQLFHPE QLITGKEDAA NNYARGHYTI GKEIIDLVLD RIRKLADQCT GLQGFLVFHS FGGGTGSGFT SLLMERLSVD YGKKSKLEFS IYPAPQVSTA VVEPYNSILT THTTLEHSDC AFMVDNEAIY DICRRNLDIE RPTYTNLNRL IGQIVSSITA SLRFDGALNV DLTEFQTNLV PYPRIHFPLA TYAPVISAEK AYHEQLSVAE ITNACFEPAN QMVKCDPRHG KYMACCLLYR GDVVPKDVNA AIATIKTKRT IQFVDWCPTG FKVGINYQPP TVVPGGDLAK VQRAVCMLSN TTAIAEAWAR LDHKFDLMYA KRAFVHWYVG EGMEEGEFSE AREDMAALEK DYEEVGVDSV EGEGEEEGEE Y
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - 产品特性
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 内毒素水平
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product Tuba1a 蛋白
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Tubulin, alpha 1a (Tuba1a) (AA 214-413) protein (His-IF2DI Tag)
Tuba1a 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % by SDS-PAGE gel analyses ELISA, WB
Tubulin, alpha 1a (Tuba1a) (AA 1-451) protein (GST tag)Tuba1a 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Tubulin, alpha 1a (Tuba1a) (AA 352-451) protein (GST tag)Tuba1a 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Tubulin, alpha 1a (Tuba1a) (AA 1-451) protein (Strep Tag)Crystallography grade Tuba1a 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Tubulin, alpha 1a (Tuba1a) protein (Myc-DYKDDDDK Tag)Tuba1a 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- TUBA1A (Tuba1a) (Tubulin, alpha 1a (Tuba1a))
- 别名
- Tuba1a (Tuba1a 产品)
- 别名
- Tuba1 Protein, B-ALPHA-1 Protein, LIS3 Protein, TUBA3 Protein, Tuba-1 Protein, TUBA3D Protein, TUBA3E Protein, wu:fb37a10 Protein, 1t Protein, ALPHA 84C Protein, Alpha-Tubulin84B Protein, BEST:LD32507 Protein, CG1913 Protein, DM1alpha Protein, DTA1 Protein, Dmel\CG1913 Protein, T Protein, TBA1_DROME Protein, Talpha1 Protein, Tub Protein, Tub 84B Protein, Tub1C Protein, Tuba84B Protein, Tubalpha1 Protein, a-tub84B Protein, a1t Protein, aTub84B Protein, alpha-Tub Protein, alpha-Tub84B Protein, alpha-tub Protein, alpha-tub84B Protein, alpha-tubulin Protein, alpha-tubulin 84B Protein, alpha1 Protein, alpha1t Protein, alpha1tub Protein, alpha1tub84B Protein, alpha84B Protein, alphaT Protein, alphaTUB Protein, alphaTUB84B Protein, alphaTub Protein, alphaTub1 Protein, alphaTub84 Protein, alphat-1 Protein, alphatub Protein, alphatub84B Protein, anon-EST:Liang-1.59 Protein, anon-EST:Liang-2.30 Protein, chr3R:2914276..2914416 Protein, clone 1.59 Protein, clone 2.30 Protein, l(3)84Bd Protein, l(3)g3 Protein, ms(3)nc33 Protein, nc33 Protein, tub Protein, tubA84B Protein, tubalpha1 Protein, tubulin Protein, TUBA1A Protein, Tuba1a Protein, Tuba2 Protein, alphatub84 Protein, b-alpha-1 Protein, lis3 Protein, tuba1a Protein, tuba1a-a Protein, tuba1a-b Protein, tuba3 Protein, tubulin, alpha 1A Protein, tubulin alpha 1a Protein, tubulin, alpha 1a Protein, tubulin alpha-1B chain Protein, tubulin alpha-1A chain Protein, tubulin alpha-3 chain-like Protein, alpha-Tubulin at 84B Protein, tubulin alpha 1a L homeolog Protein, Tuba1a Protein, TUBA1A Protein, LOC608147 Protein, LOC100392817 Protein, LOC425049 Protein, tuba1a Protein, alphaTub84B Protein, LOC100051840 Protein, LOC100340483 Protein, LOC100726777 Protein, tuba1a.L Protein
- 背景
- Tubulin alpha-1A chain (EC 3.6.5.-) (Alpha-tubulin 1) (Alpha-tubulin isotype M-alpha-1) (Tubulin alpha-1 chain) [Cleaved into: Detyrosinated tubulin alpha-1A chain],FUNCTION: Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.
- 分子量
- 50.1 kDa
- UniProt
- P68369
- 途径
- Microtubule Dynamics, M Phase
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