NUTF2 Protein (AA 1-127) (Strep Tag)
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- 抗原 See all NUTF2 蛋白
- NUTF2 (Nuclear Transport Factor 2 (NUTF2))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-127
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宿主
- 小鼠
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资源
- Tobacco (Nicotiana tabacum)
- 标记
- This NUTF2 protein is labelled with Strep Tag.
- 应用范围
- ELISA, Western Blotting (WB), SDS-PAGE (SDS)
- 序列
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MGDKPIWEQI GSSFIQHYYQ LFDNDRTQLG AIYIDASCLT WEGQQFQGKA AIVEKLSSLP FQKIQHSITA QDHQPTPDSC IISMVVGQLK ADEDPIMGFH QMFLLKNIND AWVCTNDMFR LALHNFG
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - 产品特性
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 内毒素水平
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product NUTF2 蛋白
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Nuclear Transport Factor 2 (NUTF2) (AA 1-127) protein (GST tag)
NUTF2 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Nuclear Transport Factor 2 (NUTF2) protein (Myc-DYKDDDDK Tag)NUTF2 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- NUTF2 (Nuclear Transport Factor 2 (NUTF2))
- 别名
- Nutf2 (NUTF2 产品)
- 别名
- NTF2 Protein, PP15 Protein, DDBDRAFT_0167060 Protein, DDBDRAFT_0233957 Protein, DDB_0167060 Protein, DDB_0233957 Protein, F13K9.26 Protein, F13K9_26 Protein, nuclear transport factor 2B Protein, 2700067I02Rik Protein, AI115459 Protein, AI413596 Protein, AW546000 Protein, Ntf2 Protein, Ntf3 Protein, NTF-2 Protein, ntf2 Protein, nutf2 Protein, pp15 Protein, fb40a01 Protein, wu:fb40a01 Protein, zgc:101555 Protein, nuclear transport factor 2 Protein, nuclear transport factor 2, putative Protein, nuclear transport factor 2B Protein, nuclear transport factor Nxt2 Protein, nuclear transport factor 2 L homeolog Protein, NUTF2 Protein, TP02_0050 Protein, TA13190 Protein, CC1G_11512 Protein, Bcenmc03_3881 Protein, nutf2 Protein, Nutf2 Protein, NTF2B Protein, nxt2 Protein, nutf2.L Protein
- 背景
- Nuclear transport factor 2 (NTF-2),FUNCTION: Mediates the import of GDP-bound RAN from the cytoplasm into the nucleus which is essential for the function of RAN in cargo receptor-mediated nucleocytoplasmic transport. Thereby, plays indirectly a more general role in cargo receptor-mediated nucleocytoplasmic transport. Interacts with GDP-bound RAN in the cytosol, recruits it to the nuclear pore complex via its interaction with nucleoporins and promotes its nuclear import. {ECO:0000250|UniProtKB:P61970}.
- 分子量
- 14.5 kDa
- UniProt
- P61971
- 途径
- Protein targeting to Nucleus, SARS-CoV-2 Protein Interactome
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