ABAT Protein (AA 29-500) (His tag)
-
- 抗原 See all ABAT 蛋白
- ABAT (4-Aminobutyrate Aminotransferase (ABAT))
- 蛋白类型
- Recombinant
- 产品特性
- AA 29-500
-
宿主
- 小鼠
-
资源
- Insect Cells
- 标记
- This ABAT protein is labelled with His tag.
- 应用范围
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
- 序列
-
SQAAAKVDIE FDYDGPLMKT EVPGPRSKEL MKQLNTIQNA EAVHFFCNYE ESRGNYLVDV DGNRMLDLYS QISSVPIGYN HPALAKLVQQ PQNASTFINR PALGILPPEN FVDKLQESLM SVAPRGMSQL ITMACGSCSN ENAFKTIFMW YRSKERGQRG FSKEELETCM VNQSPGCPDY SILSFMGAFH GRTMGCLATT HSKAIHKIDI PSFDWPIAPF PRLKYPLEEF TTDNQQEEAR CLEEVEDLIV KYRKKKRTVA GIIVEPIQSE GGDNHASDDF FRKLRDIARK HGCAFLVDEV QTGGGCTGKF WAHEHWGLDD PADVMTFSKK MMTGGFFHKE EFRPSAPYRI FNTWLGDPSK NLLLAEVINI IKREDLLNNV ARVGKTLLTG LLDLQAQYPQ FISRVRGRGT FCSFDTPDEA IRNKLILIAR NKGVVLGGCG DKSIRFRPTL VFRDHHAHLF LSIFSGILAD FK
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
-
- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Abat Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
-
Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product ABAT 蛋白
-
4-Aminobutyrate Aminotransferase (ABAT) (AA 1-116) protein (GST tag)
ABAT 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
4-Aminobutyrate Aminotransferase (ABAT) (AA 29-500) protein (His tag)Crystallography grade ABAT 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
4-Aminobutyrate Aminotransferase (ABAT) (Transcript Variant 3) protein (Myc-DYKDDDDK Tag)ABAT 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
4-Aminobutyrate Aminotransferase (ABAT) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)ABAT 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
4-Aminobutyrate Aminotransferase (ABAT) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)ABAT 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
-
- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
-
Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
-
- 抗原
- ABAT (4-Aminobutyrate Aminotransferase (ABAT))
- 别名
- Abat (ABAT 产品)
- 别名
- cb880 Protein, fj82a01 Protein, wu:fj82a01 Protein, BA0325 Protein, PSPTO0259 Protein, PSPTO0301 Protein, PSPTO1890 Protein, GABA-AT Protein, GABAT Protein, NPD009 Protein, GABA-T Protein, L-AIBAT Protein, 9630038C02Rik Protein, AI255750 Protein, ENSMUSG00000051226 Protein, Gabaat Protein, Gabat Protein, Gm9851 Protein, I54 Protein, Laibat Protein, X61497 Protein, beta-AlaAT Protein, 4-aminobutyrate aminotransferase Protein, 4-aminobutyrate--2-oxoglutarate transaminase Protein, GABA aminotransferase PLP-dependent PuuE Protein, 4-aminobutyrate aminotransferase, mitochondrial Protein, aspartate aminotransferase family protein Protein, 4-aminobutyrate aminotransferase S homeolog Protein, ABAT Protein, abat Protein, gabT Protein, puuE Protein, gabT-1 Protein, gabT-2 Protein, gabT-3 Protein, CNE01830 Protein, Mmwyl1_0047 Protein, CpipJ_CPIJ008729 Protein, KCR_RS04555 Protein, Bcenmc03_4909 Protein, Hden_0972 Protein, Dbac_2000 Protein, Mesil_2400 Protein, Trad_0121 Protein, abat.S Protein, Abat Protein
- 背景
- Catalyzes the conversion of gamma-aminobutyrate and L-beta-aminoisobutyrate to succinate semialdehyde and methylmalonate semialdehyde, respectively. Can also convert delta-aminovalerate and beta-alanine (By similarity). {ECO:0000250}.
- 分子量
- 54.2 kDa Including tag.
- UniProt
- P61922
- 途径
- Monocarboxylic Acid Catabolic Process
-
