Adenylosuccinate Lyase Protein (ADSL) (AA 2-484) (His tag)
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- 抗原 See all Adenylosuccinate Lyase (ADSL) 蛋白
- Adenylosuccinate Lyase (ADSL)
- 蛋白类型
- Recombinant
- 产品特性
- AA 2-484
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宿主
- 小鼠
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资源
- Insect Cells
- 标记
- This Adenylosuccinate Lyase protein is labelled with His tag.
- 应用范围
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
- 序列
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AASGDPGSAE SYRSPLAARY ASREMCFLFS DRYKFQTWRQ LWLWLAEAEQ TLGLPITDEQ IQEMKSNLNN IDFQMAAEEE KRLRHDVMAH VHTFGHCCPK AAGIIHLGAT SCYVGDNTDL IILRNAFDLL LPKLARVISR LADFAKDRAD LPTLGFTHFQ PAQLTTVGKR CCLWIQDLCM DLQNLKRVRD ELRFRGVKGT TGTQASFLQL FEGDHQKVEQ LDKMVTEKAG FKRAFIITGQ TYTRKVDIEV LSVLASLGAS VHKICTDIRL LANLKEMEEP FEKQQIGSSA MPYKRNPMRS ERCCSLARHL MALTMDPLQT ASVQWFERTL DDSANRRICL AEAFLTADTI LNTLQNISEG LVVYPKVIER RIRQELPFMA TENIIMAMVK AGGSRQDCHE KIRVLSQQAA AVVKQEGGDN DLIERIRADA YFSPIHSQLE HLLDPSSFTG RAPQQVHRFL EEEVRPLLKP YGNEMAVKAE LCL
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Adsl Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product ADSL 蛋白
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Adenylosuccinate Lyase (ADSL) (AA 1-484) protein (GST tag)
ADSL 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Adenylosuccinate Lyase (ADSL) (AA 1-484) protein (His tag)ADSL 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Adenylosuccinate Lyase (ADSL) (AA 2-484) protein (His tag)Crystallography grade ADSL 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Adenylosuccinate Lyase (ADSL) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)ADSL 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- Adenylosuccinate Lyase (ADSL)
- 别名
- Adsl (ADSL 产品)
- 别名
- fi60b04 Protein, zgc:56061 Protein, wu:fi60b04 Protein, MGC53383 Protein, MGC69239 Protein, adsL Protein, F15C21.8 Protein, F15C21_8 Protein, AMPS Protein, ASASE Protein, ASL Protein, Adl Protein, Asl Protein, purB Protein, adenylosuccinate lyase Protein, adenylosuccinate lyase L homeolog Protein, adenylosuccinase; ASL Protein, Adenylosuccinate lyase Protein, L-Aspartase-like family protein Protein, ADSL Protein, adsl Protein, adsl.L Protein, ASL Protein, purB Protein, purB,adsL Protein, Mrub_2293 Protein, MMAH_RS02975 Protein, Arnit_0089 Protein, Mesil_1058 Protein, Slip_0597 Protein, Trad_2452 Protein, Deba_0652 Protein, Toce_1174 Protein, Acear_2324 Protein, Igag_0272 Protein, Saut_2156 Protein, Fbal_1996 Protein, Ndas_2434 Protein, Ilyop_0196 Protein, Lbys_2961 Protein, MFER_RS02920 Protein, Palpr_2192 Protein, Calni_0061 Protein, Ftrac_3746 Protein, AT1G36280 Protein, Olsu_1764 Protein, Adsl Protein
- 背景
- Catalyzes two non-sequential steps in de novo AMP synthesis: converts (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate (SAICAR) to fumarate plus 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide, and thereby also contributes to de novo IMP synthesis, and converts succinyladenosine monophosphate (SAMP) to AMP and fumarate. {ECO:0000250|UniProtKB:P30566}.
- 分子量
- 55.7 kDa Including tag.
- UniProt
- P54822
- 途径
- Ribonucleoside Biosynthetic Process
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