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PTPRN2 Protein (AA 22-1015) (rho-1D4 tag)

Crystallography grade PTPRN2 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
产品编号 ABIN3117137
发货至: 中国
  • 抗原 See all PTPRN2 蛋白
    PTPRN2 (Protein tyrosine Phosphatase, Receptor Type, N Polypeptide 2 (PTPRN2))
    蛋白类型
    Recombinant
    产品特性
    AA 22-1015
    宿主
    • 6
    • 5
    • 2
    资源
    • 4
    • 4
    • 3
    • 1
    • 1
    Insect Cells
    标记
    This PTPRN2 protein is labelled with rho-1D4 tag.
    应用范围
    SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)
    序列
    AAPSSVPRGR QLPGRLGCLL EEGLCGASEA CVNDGVFGRC QKVPAMDFYR YEVSPVALQR LRVALQKLSG TGFTWQDDYT QYVMDQELAD LPKTYLRRPE ASSPARPSKH SVGSERRYSR EGGAALANAL RRHLPFLEAL SQAPASDVLA RTHTAQDRPP AEGDDRFSES ILTYVAHTSA LTYPPGSRTQ LREDLLPRTL GQLQPDELSP KVDSGVDRHH LMAALSAYAA QRPPAPPGEG SLEPQYLLRA PSRMPRPLLA PAAPQKWPSP LGDSEDPSST GDGARIHTLL KDLQRQPAEV RGLSGLELDG MAELMAGLMQ GVDHGVARGS PGRAALGESG EQADGPKATL RGDSFPDDGV QDDDDRLYQE VHRLSATLGG LLQDHGSRLL PGALPFARPL DMERKKSEHP ESSLSSEEET AGVENVKSQT YSKDLLGQQP HSEPGAAAFG ELQNQMPGPS KEEQSLPAGA QEALSDGLQL EVQPSEEEAR GYIVTDRDPL RPEEGRRLVE DVARLLQVPS SAFADVEVLG PAVTFKVSAN VQNVTTEDVE KATVDNKDKL EETSGLKILQ TGVGSKSKLK FLPPQAEQED STKFIALTLV SLACILGVLL ASGLIYCLRH SSQHRLKEKL SGLGGDPGAD ATAAYQELCR QRMATRPPDR PEGPHTSRIS SVSSQFSDGP IPSPSARSSA SSWSEEPVQS NMDISTGHMI LSYMEDHLKN KNRLEKEWEA LCAYQAEPNS SFVAQREENV PKNRSLAVLT YDHSRVLLKA ENSHSHSDYI NASPIMDHDP RNPAYIATQG PLPATVADFW QMVWESGCVV IVMLTPLAEN GVRQCYHYWP DEGSNLYHIY EVNLVSEHIW CEDFLVRSFY LKNLQTNETR TVTQFHFLSW YDRGVPSSSR SLLDFRRKVN KCYRGRSCPI IVHCSDGAGR SGTYVLIDMV LNKMAKGAKE IDIAATLEHL RDQRPGMVQT KEQFEFALTA VAEEVNAILK ALPQ
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    产品特性
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human PTPRN2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    纯化方法
    Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
    1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
    2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
    3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    纯度
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    过滤
    0.22 μm filtered
    内毒素水平
    Protein is endotoxin-free.
    质量等级
    Crystallography grade
    Top Product
    Discover our top product PTPRN2 蛋白
  • 应用备注
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    说明

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    注意事项
    Avoid repeated freeze-thaw cycles.
    储存条件
    -80 °C
    储存方法
    Store at -80°C.
    有效期
    Unlimited (if stored properly)
  • 抗原
    PTPRN2 (Protein tyrosine Phosphatase, Receptor Type, N Polypeptide 2 (PTPRN2))
    别名
    PTPRN2 (PTPRN2 产品)
    别名
    IA-2beta Protein, IAR Protein, ICAAR Protein, PTPRP Protein, R-PTP-N2 Protein, 4930425H11Rik Protein, IA2beta Protein, PTP-NP Protein, Phol Protein, mKIAA0387 Protein, phogrin Protein, protein tyrosine phosphatase, receptor type N2 Protein, protein tyrosine phosphatase, receptor type, N polypeptide 2 Protein, PTPRN2 Protein, Ptprn2 Protein
    背景
    Plays a role in vesicle-mediated secretory processes. Required for normal accumulation of secretory vesicles in hippocampus, pituitary and pancreatic islets. Required for the accumulation of normal levels of insulin-containing vesicles and preventing their degradation. Plays a role in insulin secretion in response to glucose stimuli. Required for normal accumulation of the neurotransmitters norepinephrine, dopamine and serotonin in the brain. In females, but not in males, required for normal accumulation and secretion of pituitary hormones, such as luteinizing hormone (LH) and follicle-stimulating hormone (FSH). {ECO:0000250|UniProtKB:P80560}.
    分子量
    110.2 kDa Including tag.
    UniProt
    Q92932
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