SLC1A2 Protein (AA 1-574) (Strep Tag)
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- 抗原 See all SLC1A2 蛋白
- SLC1A2 (Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 2 (SLC1A2))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-574
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宿主
- 人
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资源
- Tobacco (Nicotiana tabacum)
- 标记
- This SLC1A2 protein is labelled with Strep Tag.
- 应用范围
- Western Blotting (WB), SDS-PAGE (SDS), ELISA
- 序列
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MASTEGANNM PKQVEVRMHD SHLGSEEPKH RHLGLRLCDK LGKNLLLTLT VFGVILGAVC GGLLRLASPI HPDVVMLIAF PGDILMRMLK MLILPLIISS LITGLSGLDA KASGRLGTRA MVYYMSTTII AAVLGVILVL AIHPGNPKLK KQLGPGKKND EVSSLDAFLD LIRNLFPENL VQACFQQIQT VTKKVLVAPP PDEEANATSA VVSLLNETVT EVPEETKMVI KKGLEFKDGM NVLGLIGFFI AFGIAMGKMG DQAKLMVDFF NILNEIVMKL VIMIMWYSPL GIACLICGKI IAIKDLEVVA RQLGMYMVTV IIGLIIHGGI FLPLIYFVVT RKNPFSFFAG IFQAWITALG TASSAGTLPV TFRCLEENLG IDKRVTRFVL PVGATINMDG TALYEAVAAI FIAQMNGVVL DGGQIVTVSL TATLASVGAA SIPSAGLVTM LLILTAVGLP TEDISLLVAV DWLLDRMRTS VNVVGDSFGA GIVYHLSKSE LDTIDSQHRV HEDIEMTKTQ SIYDDMKNHR ESNSNQCVYA AHNSVIVDEC KVTLAANGKS ADCSVEEEPW KREK
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - 产品特性
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 内毒素水平
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product SLC1A2 蛋白
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Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 2 (SLC1A2) (AA 143-240) protein (His-IF2DI Tag)
SLC1A2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 2 (SLC1A2) (AA 160-239) protein (GST tag)SLC1A2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 2 (SLC1A2) (AA 1-574) protein (GST tag)SLC1A2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 2 (SLC1A2) (AA 1-572) protein (Strep Tag)Crystallography grade SLC1A2 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- SLC1A2 (Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 2 (SLC1A2))
- 别名
- SLC1A2 (SLC1A2 产品)
- 别名
- CG3159 Protein, Dmel\\CG3159 Protein, EAAT Protein, dEAAT2 Protein, dEaat2 Protein, Eaat Protein, GB16377 Protein, EAAT2 Protein, SLC1A2 Protein, Eaat2 Protein, Glt Protein, Glt-1 Protein, GLT-1 Protein, 1700091C19Rik Protein, 2900019G14Rik Protein, AI159670 Protein, GLT1 Protein, MGLT1 Protein, eaat2 Protein, fj34b12 Protein, slc1a2 Protein, wu:fj34b12 Protein, zgc:65897 Protein, Excitatory amino acid transporter 2 Protein, excitatory amino acid transporter 2 Protein, solute carrier family 1 member 2 Protein, solute carrier family 1 (glial high affinity glutamate transporter), member 2 Protein, solute carrier family 1 (glial high affinity glutamate transporter), member 2b Protein, Eaat2 Protein, Eaat-2 Protein, SLC1A2 Protein, VDBG_06180 Protein, EUBELI_01747 Protein, Trebr_0139 Protein, Slc1a2 Protein, slc1a2b Protein
- 背景
- Excitatory amino acid transporter 2 (Glutamate/aspartate transporter II) (Sodium-dependent glutamate/aspartate transporter 2) (Solute carrier family 1 member 2),FUNCTION: Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate (PubMed:7521911, PubMed:14506254, PubMed:15265858, PubMed:26690923). Functions as a symporter that transports one amino acid molecule together with two or three Na(+) ions and one proton, in parallel with the counter-transport of one K(+) ion (PubMed:14506254). Mediates Cl(-) flux that is not coupled to amino acid transport, this avoids the accumulation of negative charges due to aspartate and Na(+) symport (PubMed:14506254). Essential for the rapid removal of released glutamate from the synaptic cleft, and for terminating the postsynaptic action of glutamate (By similarity). {ECO:0000250|UniProtKB:P43006, ECO:0000269|PubMed:15265858, ECO:0000269|PubMed:26690923, ECO:0000269|PubMed:7521911}.
- 分子量
- 62.1 kDa
- UniProt
- P43004
- 途径
- Dicarboxylic Acid Transport
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