Activin A Receptor Type IB/ALK-4 Protein (AA 24-505) (rho-1D4 tag)
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- 抗原 See all Activin A Receptor Type IB/ALK-4 (ACVR1B) 蛋白
- Activin A Receptor Type IB/ALK-4 (ACVR1B) (Activin A Receptor, Type IB (ACVR1B))
- 蛋白类型
- Recombinant
- 产品特性
- AA 24-505
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宿主
- 人
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资源
- Insect Cells
- 标记
- This Activin A Receptor Type IB/ALK-4 protein is labelled with rho-1D4 tag.
- 应用范围
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
- 序列
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SGPRGVQALL CACTSCLQAN YTCETDGACM VSIFNLDGME HHVRTCIPKV ELVPAGKPFY CLSSEDLRNT HCCYTDYCNR IDLRVPSGHL KEPEHPSMWG PVELVGIIAG PVFLLFLIII IVFLVINYHQ RVYHNRQRLD MEDPSCEMCL SKDKTLQDLV YDLSTSGSGS GLPLFVQRTV ARTIVLQEII GKGRFGEVWR GRWRGGDVAV KIFSSREERS WFREAEIYQT VMLRHENILG FIAADNKDNG TWTQLWLVSD YHEHGSLFDY LNRYTVTIEG MIKLALSAAS GLAHLHMEIV GTQGKPGIAH RDLKSKNILV KKNGMCAIAD LGLAVRHDAV TDTIDIAPNQ RVGTKRYMAP EVLDETINMK HFDSFKCADI YALGLVYWEI ARRCNSGGVH EEYQLPYYDL VPSDPSIEEM RKVVCDQKLR PNIPNWWQSY EALRVMGKMM RECWYANGAA RLTALRIKKT LSQLSVQEDV KI
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human ACVR1B Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
- Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
- The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin-free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product ACVR1B 蛋白
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Activin A Receptor, Type IB (ACVR1B) (AA 150-505) (Active) protein (GST tag)
ACVR1B 宿主: 人 宿主: Insect cells (Sf21) Recombinant SDS, Func Active
Activin A Receptor, Type IB (ACVR1B) protein (Fc Tag)ACVR1B 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE
Activin A Receptor, Type IB (ACVR1B) (AA 24-126) protein (His-IF2DI Tag)ACVR1B 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Activin A Receptor, Type IB (ACVR1B) (AA 1-505) protein (GST tag)ACVR1B 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Activin A Receptor, Type IB (ACVR1B) (AA 24-126) protein (GST tag)ACVR1B 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Activin A Receptor, Type IB (ACVR1B) (Active) protein (Fc Tag)ACVR1B 宿主: 大鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE Active
Activin A Receptor, Type IB (ACVR1B) (AA 150-505) protein (His tag)Crystallography grade ACVR1B 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Activin A Receptor, Type IB (ACVR1B) (AA 24-126) protein (His tag)Crystallography grade ACVR1B 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Activin A Receptor, Type IB (ACVR1B) (AA 150-505) protein (His tag)Crystallography grade ACVR1B 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Activin A Receptor, Type IB (ACVR1B) (AA 24-126) protein (His tag)Crystallography grade ACVR1B 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- Activin A Receptor Type IB/ALK-4 (ACVR1B) (Activin A Receptor, Type IB (ACVR1B))
- 别名
- ACVR1B (ACVR1B 产品)
- 别名
- ACVR1B Protein, alk4 Protein, skr2 Protein, alk-4 Protein, xalk4 Protein, actrib Protein, acvrlk4 Protein, DKFZp469A1614 Protein, 6820432J04 Protein, ActR-IB Protein, ActRIB Protein, Acvrlk4 Protein, Alk4 Protein, SKR2 Protein, ACTRIB Protein, ACVRLK4 Protein, ALK4 Protein, Skr2 Protein, axis Protein, taram-a Protein, tarama Protein, wu:fb75f01 Protein, activin A receptor type 1B Protein, Activin receptor type-1B Protein, activin A receptor, type IB Protein, activin A receptor, type 1B Protein, activin A receptor type 1Ba Protein, ACVR1B Protein, acvr1b Protein, acv1b Protein, Acvr1b Protein, acvr1ba Protein
- 背景
- Transmembrane serine/threonine kinase activin type-1 receptor forming an activin receptor complex with activin receptor type-2 (ACVR2A or ACVR2B). Transduces the activin signal from the cell surface to the cytoplasm and is thus regulating a many physiological and pathological processes including neuronal differentiation and neuronal survival, hair follicle development and cycling, FSH production by the pituitary gland, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. Activin is also thought to have a paracrine or autocrine role in follicular development in the ovary. Within the receptor complex, type-2 receptors (ACVR2A and/or ACVR2B) act as a primary activin receptors whereas the type-1 receptors like ACVR1B act as downstream transducers of activin signals. Activin binds to type-2 receptor at the plasma membrane and activates its serine-threonine kinase. The activated receptor type-2 then phosphorylates and activates the type-1 receptor such as ACVR1B. Once activated, the type-1 receptor binds and phosphorylates the SMAD proteins SMAD2 and SMAD3, on serine residues of the C-terminal tail. Soon after their association with the activin receptor and subsequent phosphorylation, SMAD2 and SMAD3 are released into the cytoplasm where they interact with the common partner SMAD4. This SMAD complex translocates into the nucleus where it mediates activin-induced transcription. Inhibitory SMAD7, which is recruited to ACVR1B through FKBP1A, can prevent the association of SMAD2 and SMAD3 with the activin receptor complex, thereby blocking the activin signal. Activin signal transduction is also antagonized by the binding to the receptor of inhibin-B via the IGSF1 inhibin coreceptor. ACVR1B also phosphorylates TDP2. {ECO:0000269|PubMed:12364468, ECO:0000269|PubMed:12639945, ECO:0000269|PubMed:18039968, ECO:0000269|PubMed:20226172, ECO:0000269|PubMed:8196624, ECO:0000269|PubMed:9032295, ECO:0000269|PubMed:9892009}.
- 分子量
- 55.8 kDa Including tag.
- UniProt
- P36896
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