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NTSR1 Protein (AA 1-418) (rho-1D4 tag,His tag)

Crystallography grade NTSR1 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, Crys, ELISA
产品编号 ABIN3084600
发货至: 中国
  • 抗原 See all NTSR1 蛋白
    NTSR1 (Neurotensin Receptor 1 (High Affinity) (NTSR1))
    蛋白类型
    Recombinant
    产品特性
    AA 1-418
    宿主
    • 3
    • 3
    资源
    • 3
    • 1
    • 1
    • 1
    Insect Cells
    标记
    This NTSR1 protein is labelled with rho-1D4 tag,His tag.
    应用范围
    SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys), ELISA
    序列
    HHHHHHRLNSSAPGT PGTPAADPFQ RAQAGLEEAL LAPGFGNASG NASERVLAAP SSELDVNTDI YSKVLVTAVY LALFVVGTVG NTVTAFTLAR KKSLQSLQST VHYHLGSLAL SDLLTLLLAM PVELYNFIWV HHPWAFGDAG CRGYYFLRDA CTYATALNVA SLSVERYLAI CHPFKAKTLM SRSRTKKFIS AIWLASALLA VPMLFTMGEQ NRSADGQHAG GLVCTPTIHT ATVKVVIQVN TFMSFIFPMV VISVLNTIIA NKLTVMVRQA AEQGQVCTVG GEHSTFSMAI EPGRVQALRH GVRVLRAVVI AFVVCWLPYH VRRLMFCYIS DEQWTPFLYD FYHYFYMVTN ALFYVSSTIN PILYNLVSAN FRHIFLATLA CLCPVWRRRR KRPAFSRKAD SVSSNHTLSS NATRETLYGSSGTETSQVAPA
    Sequence including N-terminal His-tag and C-terminal Rho1D4 tag.
    产品特性
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human NTSR1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    纯化方法
    The protein is purified from the cleared cell lysate using Rho1D4 capture materials. Eluate fractions are analyzed by SDS-PAGE. Protein containing fractions are subjected to a second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    纯度
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    过滤
    0.22 μm filtered
    内毒素水平
    Protein is endotoxin-free.
    质量等级
    Crystallography grade
  • 应用备注
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    说明

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    In solution (50 mM Hepes, pH8.0; 200 mM NaCl; 10% Glycerol; 0.1% n-Decyl-beta-maltoside; 0.01% cholesterolhemisuccinate; 0.01% CHAPSO)
    注意事项
    Avoid repeated freeze-thaw cycles.
    储存条件
    -80 °C
    储存方法
    Store at -80°C.
    有效期
    Unlimited (if stored properly)
  • 抗原
    NTSR1 (Neurotensin Receptor 1 (High Affinity) (NTSR1))
    别名
    NTSR1 (NTSR1 产品)
    别名
    NTSR1 Protein, Ntsr Protein, NT-1R Protein, NTR-1 Protein, NTR1 Protein, NTR Protein, neurotensin receptor 1 Protein, neurotensin receptor 1 (high affinity) Protein, NTSR1 Protein, Ntsr1 Protein
    背景
    G-protein coupled receptor for the tridecapeptide neurotensin (NTS) (PubMed:8381365, PubMed:21725197, PubMed:23140271). Signaling is effected via G proteins that activate a phosphatidylinositol-calcium second messenger system. Signaling leads to the activation of downstream MAP kinases and protects cells against apoptosis (PubMed:21725197). {ECO:0000269|PubMed:21725197, ECO:0000269|PubMed:23140271, ECO:0000269|PubMed:8381365}.
    分子量
    48.1 kDa Including tag.
    UniProt
    P30989
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