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Rho-related GTP-binding protein Protein (RhO (pan)) (AA 1-348)

Crystallography grade RhO (pan) 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, Crys, ELISA, WB
产品编号 ABIN3112144
发货至: 中国
  • 抗原 See all Rho-related GTP-binding protein (RhO (pan)) products
    Rho-related GTP-binding protein (RhO (pan))
    蛋白类型
    Recombinant
    产品特性
    AA 1-348
    宿主
    • 1
    • 1
    • 1
    资源
    • 2
    • 1
    Insect Cells
    应用范围
    SDS-PAGE (SDS), Crystallization (Crys), ELISA, Western Blotting (WB)
    序列
    MNGTEGPNFY VPFSNATGVV RSPFEYPQYY LAEPWQFSML AAYMFLLIVL GFPINFLTLY VTVQHKKLRT PLNYILLNLA VADLFMVLGG FTSTLYTSLH GYFVFGPTGC NLEGFFATLG GEIALWSLVV LAIERYVVVC KPMSNFRFGE NHAIMGVAFT WVMALACAAP PLAGWSRYIP EGLQCSCGID YYTLKPEVNN ESFVIYMFVV HFTIPMIIIF FCYGQLVFTV KEAAAQQQES ATTQKAEKEV TRMVIIMVIA FLICWVPYAS VAFYIFTHQG SNFGPIFMTI PAFFAKSAAI YNPVIYIMMN KQFRNCMLTT ICCGKNPLGD DEASATVSKT ETSQVAPA
    产品特性
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human RHO Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    纯化方法
    The protein is purified from the cleared cell lysate using Rho1D4 capture materials.Eluate fractions are analyzed by SDS-PAGE.Protein containing fractions are subjected to a second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    纯度
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    过滤
    0.22 μm filtered
    内毒素水平
    Protein is endotoxin-free.
    质量等级
    Crystallography grade
  • 应用备注
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    说明

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    40 mM Hepes, pH 6.8, 10 mM KCl, 130 mM NaCl,3 mM MgCl2, 2 mM CaCl2, 0,1 mM EDTA, 1 mM DTT, Leupeptin
    注意事项
    Avoid repeated freeze-thaw cycles.
    储存条件
    -80 °C
    储存方法
    Store at -80°C.
    有效期
    Unlimited (if stored properly)
  • 抗原
    Rho-related GTP-binding protein (RhO (pan))
    别名
    rho (RhO (pan) 产品)
    物质类
    Chemical
    背景
    Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal.
    分子量
    40.1 kDa Including tag.
    UniProt
    P08100
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