B4GALT4 Protein (AA 1-344) (Strep Tag)
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- 抗原 See all B4GALT4 蛋白
- B4GALT4 (UDP-Gal:betaGlcNAc beta 1,4- Galactosyltransferase, Polypeptide 4 (B4GALT4))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-344
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宿主
- 人
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资源
- Tobacco (Nicotiana tabacum)
- 标记
- This B4GALT4 protein is labelled with Strep Tag.
- 应用范围
- ELISA, Western Blotting (WB), SDS-PAGE (SDS)
- 序列
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MGFNLTFHLS YKFRLLLLLT LCLTVVGWAT SNYFVGAIQE IPKAKEFMAN FHKTLILGKG KTLTNEASTK KVELDNCPSV SPYLRGQSKL IFKPDLTLEE VQAENPKVSR GRYRPQECKA LQRVAILVPH RNREKHLMYL LEHLHPFLQR QQLDYGIYVI HQAEGKKFNR AKLLNVGYLE ALKEENWDCF IFHDVDLVPE NDFNLYKCEE HPKHLVVGRN STGYRLRYSG YFGGVTALSR EQFFKVNGFS NNYWGWGGED DDLRLRVELQ RMKISRPLPE VGKYTMVFHT RDKGNEVNAE RMKLLHQVSR VWRTDGLSSC SYKLVSVEHN PLYINITVDF WFGA
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - 产品特性
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 内毒素水平
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product B4GALT4 蛋白
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UDP-Gal:betaGlcNAc beta 1,4- Galactosyltransferase, Polypeptide 4 (B4GALT4) (AA 35-134) protein (GST tag)
B4GALT4 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
UDP-Gal:betaGlcNAc beta 1,4- Galactosyltransferase, Polypeptide 4 (B4GALT4) (AA 1-344) protein (GST tag)B4GALT4 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
UDP-Gal:betaGlcNAc beta 1,4- Galactosyltransferase, Polypeptide 4 (B4GALT4) (AA 39-344) protein (His tag)Crystallography grade B4GALT4 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
UDP-Gal:betaGlcNAc beta 1,4- Galactosyltransferase, Polypeptide 4 (B4GALT4) (AA 39-344) protein (His tag)Crystallography grade B4GALT4 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
UDP-Gal:betaGlcNAc beta 1,4- Galactosyltransferase, Polypeptide 4 (B4GALT4) (AA 1-344) protein (Strep Tag)Crystallography grade B4GALT4 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
UDP-Gal:betaGlcNAc beta 1,4- Galactosyltransferase, Polypeptide 4 (B4GALT4) (Transcript Variant 2) protein (GST tag,His tag)B4GALT4 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
UDP-Gal:betaGlcNAc beta 1,4- Galactosyltransferase, Polypeptide 4 (B4GALT4) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)B4GALT4 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- B4GALT4 (UDP-Gal:betaGlcNAc beta 1,4- Galactosyltransferase, Polypeptide 4 (B4GALT4))
- 别名
- B4GALT4 (B4GALT4 产品)
- 别名
- B4Gal-T4 Protein, beta4Gal-T4 Protein, MGC84436 Protein, 9130402O08Rik Protein, B4galt-IV Protein, beta4GalT-IV Protein, b4Gal-T4 Protein, beta-1,4-galactosyltransferase 4 Protein, UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 4 S homeolog Protein, UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 4 Protein, B4GALT4 Protein, b4galt4.S Protein, B4galt4 Protein
- 背景
- Beta-1,4-galactosyltransferase 4 (Beta-1,4-GalTase 4) (Beta4Gal-T4) (b4Gal-T4) (EC 2.4.1.-) (Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase) (Lactotriaosylceramide beta-1,4-galactosyltransferase) (EC 2.4.1.275) (N-acetyllactosamine synthase) (EC 2.4.1.90) (Nal synthase) (UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 4) (UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 4),FUNCTION: Galactose (Gal) transferase involved in the synthesis of terminal N-acetyllactosamine (LacNac) unit present on glycan chains of glycoproteins and glycosphingolipids (PubMed:9792633, PubMed:17690104, PubMed:12511560, PubMed:32827291). Catalyzes the transfer of Gal residue via a beta1->4 linkage from UDP-Gal to the non-reducing terminal N-acetyl glucosamine 6-O-sulfate (6-O-sulfoGlcNAc) in the linearly growing chain of both N- and O-linked keratan sulfate proteoglycans. Cooperates with B3GNT7 N-acetyl glucosamine transferase and CHST6 and CHST1 sulfotransferases to construct and elongate mono- and disulfated disaccharide units [->3Galbeta1->4(6-sulfoGlcNAcbeta)1->] and [->3(6-sulfoGalbeta)1->4(6-sulfoGlcNAcbeta)1->] within keratan sulfate polymer (PubMed:17690104). Transfers Gal residue via a beta1->4 linkage to terminal 6-O-sulfoGlcNAc within the LacNac unit of core 2 O-glycans forming 6-sulfo-sialyl-Lewis X (sLex). May contribute to the generation of sLex epitope on mucin-type glycoproteins that serve as ligands for SELL/L-selectin, a major regulator of leukocyte migration (PubMed:12511560). In the biosynthesis pathway of neolacto-series glycosphingolipids, transfers Gal residue via a beta1->4 linkage to terminal GlcNAc of a lactotriaosylceramide (Lc3Cer) acceptor to form a neolactotetraosylceramide (PubMed:9792633). {ECO:0000269|PubMed:12511560, ECO:0000269|PubMed:17690104, ECO:0000269|PubMed:9792633}.
- 分子量
- 40.0 kDa
- UniProt
- O60513
- 途径
- Glycosaminoglycan Metabolic Process
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