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G6PC2 Protein (AA 1-355) (rho-1D4 tag)

Crystallography grade G6PC2 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, Crys, ELISA, SDS
产品编号 ABIN3107268
发货至: 中国
  • 抗原 See all G6PC2 products
    G6PC2 (Glucose-6-Phosphatase, Catalytic, 2 (G6PC2))
    蛋白类型
    Recombinant
    产品特性
    AA 1-355
    宿主
    • 1
    • 1
    资源
    • 1
    • 1
    Insect Cells
    标记
    This G6PC2 protein is labelled with rho-1D4 tag.
    应用范围
    Western Blotting (WB), Crystallization (Crys), ELISA, SDS-PAGE (SDS)
    序列
    MDFLHRNGVL IIQHLQKDYR AYYTFLNFMS NVGDPRNIFF IYFPLCFQFN QTVGTKMIWV AVIGDWLNLI FKWILFGHRP YWWVQETQIY PNHSSPCLEQ FPTTCETGPG SPSGHAMGAS CVWYVMVTAA LSHTVCGMDK FSITLHRLTW SFLWSVFWLI QISVCISRVF IATHFPHQVI LGVIGGMLVA EAFEHTPGIQ TASLGTYLKT NLFLFLFAVG FYLLLRVLNI DLLWSVPIAK KWCANPDWIH IDTTPFAGLV RNLGVLFGLG FAINSEMFLL SCRGGNNYTL SFRLLCALTS LTILQLYHFL QIPTHEEHLF YVLSFCKSAS IPLTVVAFIP YSVHMLMKQS GKKSQGSSGTETSQV APA
    Sequence including C-terminal rho1D4 tag.
    产品特性
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human G6PC2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).
    • Tag Location: C-terminal rho1D4 tag.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    纯化方法
    The protein is purified in three steps from baculovirus infected SF9 insect cells. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot. The best performing detergents - Fos-Choline 14 and DDM - are used for solubilization and G6PC2 is purified via the C-terminal rho1D4 tag. Eluate fractions are analyzed by Western blot. Protein containing fractions of the best purification are subjected to a second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    纯度
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    过滤
    0.22 μm filtered
    内毒素水平
    Protein is endotoxin-free.
    质量等级
    Crystallography grade
  • 应用备注
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    说明

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    注意事项
    Avoid repeated freeze-thaw cycles.
    储存条件
    -80 °C
    储存方法
    Store at -80°C.
    有效期
    Unlimited (if stored properly)
  • 抗原
    G6PC2 (Glucose-6-Phosphatase, Catalytic, 2 (G6PC2))
    别名
    G6PC2 (G6PC2 产品)
    别名
    IGRP Protein, G6pc-rs Protein, Igrp Protein, g6pc2 Protein, igrp Protein, glucose-6-phosphatase catalytic subunit 2 Protein, glucose-6-phosphatase, catalytic, 2 Protein, glucose-6-phosphatase, catalytic, 2 S homeolog Protein, G6PC2 Protein, G6pc2 Protein, g6pc2.S Protein
    背景
    May hydrolyze glucose-6-phosphate to glucose in the endoplasmic reticulum. May be responsible for glucose production through glycogenolysis and gluconeogenesis (By similarity). {ECO:0000250}.
    分子量
    41.8 kDa Including tag.
    UniProt
    Q9NQR9
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