EBP Protein (AA 2-230) (rho-1D4 tag)
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- 抗原 See all EBP 蛋白
- EBP (Emopamil Binding Protein (Sterol Isomerase) (EBP))
- 蛋白类型
- Recombinant
- 产品特性
- AA 2-230
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宿主
- 人
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资源
- Insect Cells
- 标记
- This EBP protein is labelled with rho-1D4 tag.
- 应用范围
- SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys), ELISA
- 序列
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TTNAGPLHPY WPQHLRLDNF VPNDRPTWHI LAGLFSVTGV LVVTTWLLSG RAAVVPLGTW RRLSLCWFAV CGFIHLVIEG WFVLYYEDLL GDQAFLSQLW KEYAKGDSRY ILGDNFTVCM ETITACLWGP LSLWVVIAFL RQHPLRFILQ LVVSVGQIYG DVLYFLTEHR DGFQHGELGH PLYFWFYFVF MNALWLVLPG VLVLDAVKHL THAQSTLDAK ATKAKSKKN
GSSGTETSQVAPA
C-terminal Rho-tag - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human EBP Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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The protein is purified from the cleared cell lysate using Rho1D4-tag capture materials. Eluate fractions are analyzed by SDS-PAGE.
Protein containing fractions are subjected to a second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot. - 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin-free.
- 质量等级
- Crystallography grade
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Emopamil Binding Protein (Sterol Isomerase) (EBP) (AA 2-230) protein (rho-1D4 tag)
Crystallography grade EBP 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, Crys, ELISA
Emopamil Binding Protein (Sterol Isomerase) (EBP) protein (Myc-DYKDDDDK Tag)EBP 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- in solution (20 mM Hepes, pH 7.5, 100 mM NaCl, 0.1 % DDM)
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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