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Aquaporin 4 Protein (AQP4) (AA 1-323) (rho-1D4 tag,His tag)

Crystallography grade AQP4 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
产品编号 ABIN3089096
发货至: 中国
  • 抗原 See all Aquaporin 4 (AQP4) 蛋白
    Aquaporin 4 (AQP4)
    蛋白类型
    Recombinant
    产品特性
    AA 1-323
    宿主
    • 8
    • 3
    • 2
    • 1
    • 1
    资源
    • 8
    • 1
    • 1
    • 1
    Insect Cells
    标记
    This Aquaporin 4 protein is labelled with rho-1D4 tag,His tag.
    应用范围
    Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
    序列
    MTETSQVAPA GSSGHHHHHH SDRPTARRWG KCGPLCTREN IMVAFKGVWT QAFWKAVTAE FLAMLIFVLL SLGSTINWGG TEKPLPVDMV LISLCFGLSI ATMVQCFGHI SGGHINPAVT VAMVCTRKIS IAKSVFYIAA QCLGAIIGAG ILYLVTPPSV VGGLGVTMVH GNLTAGHGLL VELIITFQLV FTIFASCDSK RTDVTGSIAL AIGFSVAIGH LFAINYTGAS MNPARSFGPA VIMGNWENHW IYWVGPIIGA VLAGGLYEYV FCPDVEFKRR FKEAFSKAAQ QTKGSYMEVE DNRSQVETDD LILKPGVVHV IDVDRGEEKK GKDQSGEVLS SV
    Sequence including N-terminal Rho1D4 tag and N-terminal His-tag.
    产品特性
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human AQP4 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    纯化方法
    Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
    1. The protein is purified from the cleared cell lysate using His-tag capture materials.
    2. Eluate fractions are analyzed by SDS-PAGE.
    3. Protein containing fractions are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    纯度
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    过滤
    0.22 μm filtered
    内毒素水平
    Protein is endotoxin-free.
    质量等级
    Crystallography grade
  • 应用备注
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    说明

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    In solution (20 mM Hepes, pH 8.0, 150 mM NaCl, 0,8 % Octylglucopyranoside)
    注意事项
    Avoid repeated freeze-thaw cycles.
    储存条件
    -80 °C
    储存方法
    Store at -80°C.
    有效期
    Unlimited (if stored properly)
  • 抗原
    Aquaporin 4 (AQP4)
    别名
    AQP4 (AQP4 产品)
    别名
    zgc:100922 Protein, si:ch211-192k9.1 Protein, AQP4 Protein, LOC100232616 Protein, HMIWC2 Protein, MIWC Protein, WCH4 Protein, AQP-4 Protein, AQP4.M23 Protein, MIWC2 Protein, Miwc Protein, aquaporin-4 Protein, aquaporin 4 Protein, aquaporin 4 L homeolog Protein, aqp4 Protein, AQP4 Protein, aqp4.L Protein, Aqp4 Protein
    背景
    Forms a water-specific channel. Osmoreceptor which regulates body water balance and mediates water flow within the central nervous system.
    分子量
    36.8 kDa Including tag.
    UniProt
    P55087
    途径
    Sensory Perception of Sound
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