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WEE1 Protein (AA 1-646) (His tag)

Crystallography grade WEE1 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS, Crys
产品编号 ABIN3096406
发货至: 中国
  • 抗原 See all WEE1 蛋白
    WEE1 (WEE1 Homolog (S. Pombe) (WEE1))
    蛋白类型
    Recombinant
    产品特性
    AA 1-646
    宿主
    • 6
    • 1
    资源
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Insect Cells
    标记
    This WEE1 protein is labelled with His tag.
    应用范围
    Western Blotting (WB), ELISA, SDS-PAGE (SDS), Crystallization (Crys)
    序列
    MSFLSRQQPP PPRRAGAACT LRQKLIFSPC SDCEEEEEEE EEEGSGHSTG EDSAFQEPDS PLPPARSPTE PGPERRRSPG PAPGSPGELE EDLLLPGACP GADEAGGGAE GDSWEEEGFG SSSPVKSPAA PYFLGSSFSP VRCGGPGDAS PRGCGARRAG EGRRSPRPDH PGTPPHKTFR KLRLFDTPHT PKSLLSKARG IDSSSVKLRG SSLFMDTEKS GKREFDVRQT PQVNINPFTP DSLLLHSSGQ CRRRKRTYWN DSCGEDMEAS DYELEDETRP AKRITITESN MKSRYTTEFH ELEKIGSGEF GSVFKCVKRL DGCIYAIKRS KKPLAGSVDE QNALREVYAH AVLGQHSHVV RYFSAWAEDD HMLIQNEYCN GGSLADAISE NYRIMSYFKE AELKDLLLQV GRGLRYIHSM SLVHMDIKPS NIFISRTSIP NAASEEGDED DWASNKVMFK IGDLGHVTRI SSPQVEEGDS RFLANEVLQE NYTHLPKADI FALALTVVCA AGAEPLPRNG DQWHEIRQGR LPRIPQVLSQ EFTELLKVMI HPDPERRPSA MALVKHSVLL SASRKSAEQL RIELNAEKFK NSLLQKELKK AQMAKAAAEE RALFTDRMAT RSTTQSNRTS RLIGKKMNRS VSLTIY
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    产品特性
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human WEE1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    纯化方法
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    纯度
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    过滤
    0.22 μm filtered
    内毒素水平
    Protein is endotoxin free.
    质量等级
    Crystallography grade
    Top Product
    Discover our top product WEE1 蛋白
  • 应用备注
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    说明

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    注意事项
    Avoid repeated freeze-thaw cycles.
    储存条件
    -80 °C
    储存方法
    Store at -80°C.
    有效期
    Unlimited (if stored properly)
  • 抗原
    WEE1 (WEE1 Homolog (S. Pombe) (WEE1))
    别名
    WEE1 (WEE1 产品)
    别名
    WEE1A Protein, WEE1hu Protein, ATWEE1 Protein, F22D16.3 Protein, F22D16_3 Protein, WEE1 kinase homolog Protein, WEE1-KINASE Protein, WEE1 Protein, Xe-Wee1B Protein, XeWee1B Protein, wee1-b Protein, wee1a Protein, wee2-a Protein, xe-wee1a Protein, xwee1 Protein, wee1 Protein, wee1b Protein, wee1hu Protein, xwee2 Protein, GB19689 Protein, Wee1A Protein, fa20h03 Protein, wu:fa20h03 Protein, zgc:136514 Protein, WEE1 G2 checkpoint kinase Protein, Wee1 kinase Protein, WEE1-like kinase Protein, WEE1 G2 checkpoint kinase S homeolog Protein, wee1 homolog Protein, WEE1 homolog 2 Protein, wee1-like protein kinase Protein, WEE 1 homolog 1 (S. pombe) Protein, M phase inhibitor protein kinase Wee1 Protein, WEE1 Protein, Wee1 Protein, wee1.S Protein, wee1-a Protein, wee2 Protein, LOC551677 Protein, wee1 Protein
    背景
    Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.
    分子量
    72.6 kDa Including tag.
    UniProt
    P30291
    途径
    Cell Division Cycle, Mitotic G1-G1/S Phases, M Phase
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