THO Complex 4 Protein (THOC4) (AA 2-257) (His tag,Strep Tag)
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- 抗原 See all THO Complex 4 (THOC4) 蛋白
- THO Complex 4 (THOC4)
- 蛋白类型
- Recombinant
- 产品特性
- AA 2-257
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This THO Complex 4 protein is labelled with His tag,Strep Tag.
- 应用范围
- ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
- 序列
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WSHPQFEK GSSGENLYFQGSADKMDMSLDD IIKLNRSQRG GRGGGRGRGR AGSQGGRGGG AQAAARVNRG GGPIRNRPAI ARGAAGGGGR NRPAPYSRPK QLPDKWQHDL FDSGFGGGAG VETGGKLLVS NLDFGVSDAD IQELFAEFGT LKKAAVHYDR SGRSLGTADV HFERKADALK AMKQYNGVPL DGRPMNIQLV TSQIDAQRRP AQSVNRGGMT RNRGAGGFGG GGGTRRGTRG GARGRGRGAG RNSKQQLSAE ELDAQLDAYN ARMDTSHHHHHH
N-terminal-Strep tag with TEV cleavage site; C-terminal His-tag - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human ALYREF Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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The protein is purified from the cleared cell lysate using EDTA and DTT resistant His-tag capture materials. Eluate fractions are analyzed by SDS-PAGE.
Protein containing fractions are subjected to a second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot. - 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Endotoxin has not been removed. Please contact us if you require endotoxin removal.
- 质量等级
- Crystallography grade
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THO Complex 4 (THOC4) (AA 106-193) protein (GST tag)
THOC4 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
THO Complex 4 (THOC4) (AA 2-255) protein (His tag)Crystallography grade THOC4 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- in solution (50 mM Hepes, pH 7.5, 150 mM NaCl)
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- THO Complex 4 (THOC4)
- 别名
- ALYREF (THOC4 产品)
- 别名
- THOC4 Protein, Aly Protein, ALY Protein, ALY/REF Protein, BEF Protein, REF Protein, tho4 Protein, thoc4 Protein, zgc:171753 Protein, Tho4-A Protein, alyref-a Protein, thoc4-a Protein, REF1 Protein, Refbp1 Protein, Thoc4 Protein, Tho4 Protein, Aly/REF export factor Protein, THO complex 4 Protein, Aly/REF export factor L homeolog Protein, ALYREF Protein, Thoc4 Protein, alyref Protein, alyref.L Protein, Alyref Protein
- 背景
- Export adapter involved in nuclear export of spliced and unspliced mRNA. Binds mRNA which is thought to be transferred to the NXF1-NXT1 heterodimer for export (TAP/NFX1 pathway). Component of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm. TREX recruitment occurs via an interaction between ALYREF/THOC4 and the cap-binding protein NCBP1. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production, ALYREF/THOC4 mediates the recruitment of the TREX complex to the intronless viral mRNA. Required for TREX complex assembly and for linking DDX39B to the cap-binding complex (CBC). In conjunction with THOC5 functions in NXF1-NXT1 mediated nuclear export of HSP70 mRNA, both proteins enhance the RNA binding activity of NXF1 and are required for NXF1 localization to the nuclear rim. Involved in the nuclear export of intronless mRNA, proposed to be recruited to intronless mRNA by ATP-bound DDX39B. Involved in transcription elongation and genome stability. {ECO:0000269|PubMed:25662211}., Acts as chaperone and promotes the dimerization of transcription factors containing basic leucine zipper (bZIP) domains and thereby promotes transcriptional activation.
- 分子量
- 27.7 kDa Including tag.
- UniProt
- Q86V81
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