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SFMBT1 Protein (AA 1-866) (His tag)

Crystallography grade SFMBT1 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
产品编号 ABIN3095579
发货至: 中国
  • 抗原 See all SFMBT1 蛋白
    SFMBT1 (Scm-Like with Four Mbt Domains 1 (SFMBT1))
    蛋白类型
    Recombinant
    产品特性
    AA 1-866
    宿主
    • 2
    • 1
    资源
    • 2
    • 1
    Insect Cells
    标记
    This SFMBT1 protein is labelled with His tag.
    应用范围
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    序列
    MNGEQQLDAD AGSGMEEVEL SWEDYLEETG STAVPYGSFK HVDTRLQNGF APGMKLEVAV RTDPETYWVA TVITTCEQLL LLRYDGYGED RRADFWCDIR KADLYPIGWC EQNKKTLEAP EGIRDKVSDW DEFLRQTLIG ACSPPVPLLE GLRNGRNPLD LIAPGSRLEC QAFQDSLSTW IVTVVENIGG RLKLRYEGLE SSDNYEHWLY YLDPFLHHVG WAAQQGYELQ PPSAIRHLKN EAEWQEILAK VKEEEEEPLP SYLFKDKQVI GIHTFSVNMK LEAVDPWSPF GISPATVVKV FDEKYFLVEM DDLRPENHAR RSFVCHADSP GIFPVQWSLK NGLHISPPPG YPSQDFDWAD YLKQCGAEAA PQRCFPPLIS EHEFKENMKL EAVNPILPEE VCVATITAVR GSYLWLQLEG SKKPIPECIV SVESMDIFPL GWCETNGHPL STPRRARVYK QRKIAVVQPE KQVPSSRTVH EGLRNQELNS TESVMINGKY CCPKIYFNHR CFSGPYLNKG RIAELPQCVG PGNCVLVLRE VLTLLINAAY KPSRVLRELQ LDKDSVWHGC GEVLKAKYKG KSYRATVEIV KTADRVTEFC RQTCIKLECC PNLFGPRMVL DKCSENCSVL TKTKYTHYYG KKKNKRIGRP PGGHSNLACA LKKASKRRKR RKNVFVHKKK RSSASVDNTP AGSPQGSGGE DEDDPDEGDD DSLSEGSTSE QQDELQEESE MSEKKSCSSS PTQSEISTSL PPDRQRRKRE LRTFSFSDDE NKPPSPKEIR IEVAERLHLD SNPLKWSVAD VVRFIRSTDC APLARIFLDQ EIDGQALLLL TLPTVQECMD LKLGPAIKLC HHIERIKFAF YEQFAN
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    产品特性
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human SFMBT1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    纯化方法
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    纯度
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    过滤
    0.22 μm filtered
    内毒素水平
    Protein is endotoxin free.
    质量等级
    Crystallography grade
    Top Product
    Discover our top product SFMBT1 蛋白
  • 应用备注
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    说明

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    注意事项
    Avoid repeated freeze-thaw cycles.
    储存条件
    -80 °C
    储存方法
    Store at -80°C.
    有效期
    Unlimited (if stored properly)
  • 抗原
    SFMBT1 (Scm-Like with Four Mbt Domains 1 (SFMBT1))
    别名
    SFMBT1 (SFMBT1 产品)
    别名
    SFMBT1 Protein, wu:fc12f05 Protein, RU1 Protein, SFMBT Protein, hSFMBT Protein, 4930442N21Rik Protein, 9330180L21Rik Protein, AA536974 Protein, Sfmbt Protein, Smr Protein, Scm-like with four mbt domains 1 Protein, Scm like with four mbt domains 1 Protein, SFMBT1 Protein, sfmbt1 Protein, Sfmbt1 Protein
    背景
    Histone-binding protein, which is part of various corepressor complexes. Mediates the recruitment of corepressor complexes to target genes, followed by chromatin compaction and repression of transcription. Plays a role during myogenesis: required for the maintenance of undifferentiated states of myogenic progenitor cells via interaction with MYOD1. Interaction with MYOD1 leads to the recruitment of associated corepressors and silencing of MYOD1 target genes. Part of the SLC complex in germ cells, where it may play a role during spermatogenesis. {ECO:0000269|PubMed:17599839, ECO:0000269|PubMed:23349461, ECO:0000269|PubMed:23592795}.
    分子量
    99.1 kDa Including tag.
    UniProt
    Q9UHJ3
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