SEMA5A Protein (AA 23-1074) (rho-1D4 tag)
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- 抗原 See all SEMA5A 蛋白
- SEMA5A (Sema Domain, Seven thrombospondin Repeats (Type 1 and Type 1-Like), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 5A (SEMA5A))
- 蛋白类型
- Recombinant
- 产品特性
- AA 23-1074
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宿主
- 人
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资源
- Insect Cells
- 标记
- This SEMA5A protein is labelled with rho-1D4 tag.
- 应用范围
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
- 序列
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EAQGTTQCQR TEHPVISYKE IGPWLREFRA KNAVDFSQLT FDPGQKELVV GARNYLFRLQ LEDLSLIQAV EWECDEATKK ACYSKGKSKE ECQNYIRVLL VGGDRLFTCG TNAFTPVCTN RSLSNLTEIH DQISGMARCP YSPQHNSTAL LTAGGELYAA TAMDFPGRDP AIYRSLGILP PLRTAQYNSK WLNEPNFVSS YDIGNFTYFF FRENAVEHDC GKTVFSRAAR VCKNDIGGRF LLEDTWTTFM KARLNCSRPG EVPFYYNELQ STFFLPELDL IYGIFTTNVN SIAASAVCVF NLSAIAQAFS GPFKYQENSR SAWLPYPNPN PHFQCGTVDQ GLYVNLTERN LQDAQKFILM HEVVQPVTTV PSFMEDNSRF SHVAVDVVQG REALVHIIYL ATDYGTIKKV RVPLNQTSSS CLLEEIELFP ERRREPIRSL QILHSQSVLF VGLREHVVKI PLKRCQFYRT RSTCIGAQDP YCGWDVVMKK CTSLEESLSM TQWEQSISAC PTRNLTVDGH FGVWSPWTPC THTDGSAVGS CLCRTRSCDS PAPQCGGWQC EGPGMEIANC SRNGGWTPWT SWSPCSTTCG IGFQVRQRSC SNPTPRHGGR VCVGQNREER YCNEHLLCPP HMFWTGWGPW ERCTAQCGGG IQARRRICEN GPDCAGCNVE YQSCNTNPCP ELKKTTPWTP WTPVNISDNG GHYEQRFRYT CKARLADPNL LEVGRQRIEM RYCSSDGTSG CSTDGLSGDF LRAGRYSAHT VNGAWSAWTS WSQCSRDCSR GIRNRKRVCN NPEPKYGGMP CLGPSLEYQE CNILPCPVDG VWSCWSPWTK CSATCGGGHY MRTRSCSNPA PAYGGDICLG LHTEEALCNT QPCPESWSEW SDWSECEASG VQVRARQCIL LFPMGSQCSG NTTESRPCVF DSNFIPEVSV ARSSSVEEKR CGEFNMFHMI AVGLSSSILG CLLTLLVYTY CQRYQQQSHD ATVIHPVSPA PLNTSITNHI NKLDKYDSVE AIKAFNKNNL ILEERNKYFN PHLTGKTYSN AYFTDLNNYD EY
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human SEMA5A Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
- Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
- The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin-free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product SEMA5A 蛋白
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Sema Domain, Seven thrombospondin Repeats (Type 1 and Type 1-Like), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 5A (SEMA5A) (AA 393-499) protein (GST tag)
SEMA5A 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Sema Domain, Seven thrombospondin Repeats (Type 1 and Type 1-Like), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 5A (SEMA5A) protein (His tag)SEMA5A 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 85 % as determined by SDS-PAGE
Sema Domain, Seven thrombospondin Repeats (Type 1 and Type 1-Like), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 5A (SEMA5A) (AA 23-968) protein (His tag)Crystallography grade SEMA5A 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Sema Domain, Seven thrombospondin Repeats (Type 1 and Type 1-Like), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 5A (SEMA5A) (AA 22-971) protein (His tag)Crystallography grade SEMA5A 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Sema Domain, Seven thrombospondin Repeats (Type 1 and Type 1-Like), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 5A (SEMA5A) (AA 23-1074) protein (rho-1D4 tag)Crystallography grade SEMA5A 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Sema Domain, Seven thrombospondin Repeats (Type 1 and Type 1-Like), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 5A (SEMA5A) (AA 22-1077) protein (rho-1D4 tag)Crystallography grade SEMA5A 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- SEMA5A (Sema Domain, Seven thrombospondin Repeats (Type 1 and Type 1-Like), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 5A (SEMA5A))
- 别名
- SEMA5A (SEMA5A 产品)
- 别名
- wu:fa95e03 Protein, wu:fi41a04 Protein, SEMAF Protein, semF Protein, 5930434A13 Protein, 9130201M22Rik Protein, AI464145 Protein, Semaf Protein, semaphorin 5A Protein, sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A Protein, semaphorin-5A Protein, SEMA5A Protein, sema5a Protein, Sema5a Protein, LOC100516117 Protein
- 背景
- Bifunctional axonal guidance cue regulated by sulfated proteoglycans, attractive effects result from interactions with heparan sulfate proteoglycans (HSPGs), while the inhibitory effects depend on interactions with chondroitin sulfate proteoglycans (CSPGs) (By similarity). Ligand for receptor PLXNB3. In glioma cells, SEMA5A stimulation of PLXNB3 results in the disassembly of F-actin stress fibers, disruption of focal adhesions and cellular collapse as well as inhibition of cell migration and invasion through ARHGDIA-mediated inactivation of RAC1. May promote angiogenesis by increasing endothelial cell proliferation and migration and inhibiting apoptosis. {ECO:0000250, ECO:0000269|PubMed:15218527, ECO:0000269|PubMed:19850054, ECO:0000269|PubMed:20696765, ECO:0000269|PubMed:21706053}.
- 分子量
- 119.3 kDa Including tag.
- UniProt
- Q13591
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