Peroxidasin Protein (AA 27-1479) (His tag)
-
- 抗原 See all Peroxidasin (PXDN) 蛋白
- Peroxidasin (PXDN) (Peroxidasin Homolog (PXDN))
- 蛋白类型
- Recombinant
- 产品特性
- AA 27-1479
-
宿主
- 人
-
资源
- Insect Cells
- 标记
- This Peroxidasin protein is labelled with His tag.
- 应用范围
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
- 序列
-
VVAQKPGAGC PSRCLCFRTT VRCMHLLLEA VPAVAPQTSI LDLRFNRIRE IQPGAFRRLR NLNTLLLNNN QIKRIPSGAF EDLENLKYLY LYKNEIQSID RQAFKGLASL EQLYLHFNQI ETLDPDSFQH LPKLERLFLH NNRITHLVPG TFNHLESMKR LRLDSNTLHC DCEILWLADL LKTYAESGNA QAAAICEYPR RIQGRSVATI TPEELNCERP RITSEPQDAD VTSGNTVYFT CRAEGNPKPE IIWLRNNNEL SMKTDSRLNL LDDGTLMIQN TQETDQGIYQ CMAKNVAGEV KTQEVTLRYF GSPARPTFVI QPQNTEVLVG ESVTLECSAT GHPPPRISWT RGDRTPLPVD PRVNITPSGG LYIQNVVQGD SGEYACSATN NIDSVHATAF IIVQALPQFT VTPQDRVVIE GQTVDFQCEA KGNPPPVIAW TKGGSQLSVD RRHLVLSSGT LRISGVALHD QGQYECQAVN IIGSQKVVAH LTVQPRVTPV FASIPSDTTV EVGANVQLPC SSQGEPEPAI TWNKDGVQVT ESGKFHISPE GFLTINDVGP ADAGRYECVA RNTIGSASVS MVLSVNVPDV SRNGDPFVAT SIVEAIATVD RAINSTRTHL FDSRPRSPND LLALFRYPRD PYTVEQARAG EIFERTLQLI QEHVQHGLMV DLNGTSYHYN DLVSPQYLNL IANLSGCTAH RRVNNCSDMC FHQKYRTHDG TCNNLQHPMW GASLTAFERL LKSVYENGFN TPRGINPHRL YNGHALPMPR LVSTTLIGTE TVTPDEQFTH MLMQWGQFLD HDLDSTVVAL SQARFSDGQH CSNVCSNDPP CFSVMIPPND SRARSGARCM FFVRSSPVCG SGMTSLLMNS VYPREQINQL TSYIDASNVY GSTEHEARSI RDLASHRGLL RQGIVQRSGK PLLPFATGPP TECMRDENES PIPCFLAGDH RANEQLGLTS MHTLWFREHN RIATELLKLN PHWDGDTIYY ETRKIVGAEI QHITYQHWLP KILGEVGMRT LGEYHGYDPG INAGIFNAFA TAAFRFGHTL VNPLLYRLDE NFQPIAQDHL PLHKAFFSPF RIVNEGGIDP LLRGLFGVAG KMRVPSQLLN TELTERLFSM AHTVALDLAA INIQRGRDHG IPPYHDYRVY CNLSAAHTFE DLKNEIKNPE IREKLKRLYG STLNIDLFPA LVVEDLVPGS RLGPTLMCLL STQFKRLRDG DRLWYENPGV FSPAQLTQIK QTSLARILCD NADNITRVQS DVFRVAEFPH GYGSCDEIPR VDLRVWQDCC EDCRTRGQFN AFSYHFRGRR SLEFSYQEDK PTKKTRPRKI PSVGRQGEHL SNSTSAFSTR SDASGTNDFR EFVLEMQKTI TDLRTQIKKL ESRLSTTECV DAGGESHANN TKWKKDACTI CECKDGQVTC FVEACPPATC AVPVNIPGAC CPVCLQKRAE EKP
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
-
- Made in Germany - from design to production - by highly experienced protein experts.
- Human PXDN Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
-
Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product PXDN 蛋白
-
Peroxidasin Homolog (PXDN) (AA 595-702) protein (His-IF2DI Tag)
PXDN 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Peroxidasin Homolog (PXDN) (AA 1452-1561) protein (GST tag)PXDN 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Peroxidasin Homolog (PXDN) (AA 24-1475) protein (His tag)Crystallography grade PXDN 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Peroxidasin Homolog (PXDN) protein (Myc-DYKDDDDK Tag)PXDN 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
-
- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
-
In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
-
- 抗原
- Peroxidasin (PXDN) (Peroxidasin Homolog (PXDN))
- 别名
- PXDN (PXDN 产品)
- 别名
- D2S448 Protein, D2S448E Protein, MG50 Protein, PRG2 Protein, PXN Protein, VPO Protein, 2310075M15Rik Protein, C85409 Protein, E330004E07 Protein, VPO1 Protein, mKIAA0230 Protein, peroxidasin Protein, PXDN Protein, Pxdn Protein
- 背景
- Displays low peroxidase activity and is likely to participate in H(2)O(2) metabolism and peroxidative reactions in the cardiovascular system. Plays a role in extracellular matrix formation. {ECO:0000269|PubMed:18929642, ECO:0000269|PubMed:19590037}.
- 分子量
- 163.4 kDa Including tag.
- UniProt
- Q92626
-
