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PIK3R1 Protein (AA 2-724) (His tag)

Crystallography grade PI3K p85a 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, Crys, SDS
产品编号 ABIN3094436
发货至: 中国
  • 抗原 See all PIK3R1 (PI3K p85a) 蛋白
    PIK3R1 (PI3K p85a) (Phosphoinositide 3 Kinase, p85 alpha (PI3K p85a))
    蛋白类型
    Recombinant
    产品特性
    AA 2-724
    宿主
    • 5
    • 1
    资源
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Insect Cells
    标记
    This PIK3R1 protein is labelled with His tag.
    应用范围
    Western Blotting (WB), ELISA, Crystallization (Crys), SDS-PAGE (SDS)
    序列
    SAEGYQYRAL YDYKKEREED IDLHLGDILT VNKGSLVALG FSDGQEARPE EIGWLNGYNE TTGERGDFPG TYVEYIGRKK ISPPTPKPRP PRPLPVAPGS SKTEADVEQQ ALTLPDLAEQ FAPPDIAPPL LIKLVEAIEK KGLECSTLYR TQSSSNLAEL RQLLDCDTPS VDLEMIDVHV LADAFKRYLL DLPNPVIPAA VYSEMISLAP EVQSSEEYIQ LLKKLIRSPS IPHQYWLTLQ YLLKHFFKLS QTSSKNLLNA RVLSEIFSPM LFRFSAASSD NTENLIKVIE ILISTEWNER QPAPALPPKP PKPTTVANNG MNNNMSLQDA EWYWGDISRE EVNEKLRDTA DGTFLVRDAS TKMHGDYTLT LRKGGNNKLI KIFHRDGKYG FSDPLTFSSV VELINHYRNE SLAQYNPKLD VKLLYPVSKY QQDQVVKEDN IEAVGKKLHE YNTQFQEKSR EYDRLYEEYT RTSQEIQMKR TAIEAFNETI KIFEEQCQTQ ERYSKEYIEK FKREGNEKEI QRIMHNYDKL KSRISEIIDS RRRLEEDLKK QAAEYREIDK RMNSIKPDLI QLRKTRDQYL MWLTQKGVRQ KKLNEWLGNE NTEDQYSLVE DDEDLPHHDE KTWNVGSSNR NKAENLLRGK RDGTFLVRES SKQGCYACSV VVDGEVKHCV INKTATGYGF AEPYNLYSSL KELVLHYQHT SLVQHNDSLN VTLAYPVYAQ QRR
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    产品特性
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human PIK3R1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    纯化方法
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    纯度
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    过滤
    0.22 μm filtered
    内毒素水平
    Protein is endotoxin free.
    质量等级
    Crystallography grade
    Top Product
    Discover our top product PI3K p85a 蛋白
  • 应用备注
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    说明

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    注意事项
    Avoid repeated freeze-thaw cycles.
    储存条件
    -80 °C
    储存方法
    Store at -80°C.
    有效期
    Unlimited (if stored properly)
  • 抗原
    PIK3R1 (PI3K p85a) (Phosphoinositide 3 Kinase, p85 alpha (PI3K p85a))
    别名
    PIK3R1 (PI3K p85a 产品)
    别名
    PI3CG Protein, PI3K Protein, PI3Kgamma Protein, PIK3 Protein, AGM7 Protein, GRB1 Protein, p85 Protein, p85-ALPHA Protein, PI3KA Protein, AA414921 Protein, C530050K14 Protein, p50alpha Protein, p55alpha Protein, p85alpha Protein, wu:fb65b05 Protein, phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma Protein, phosphoinositide-3-kinase regulatory subunit 1 Protein, phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit beta Protein, phosphoinositide-3-kinase, regulatory subunit 1 (alpha) Protein, PIK3CG Protein, PIK3R1 Protein, Pik3r1 Protein, Pik3cb Protein, pik3r1 Protein
    背景
    Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling (PubMed:17626883, PubMed:19805105, PubMed:7518429). Modulates the cellular response to ER stress by promoting nuclear translocation of XBP1 isoform 2 in a ER stress-and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (PubMed:20348923). {ECO:0000269|PubMed:17626883, ECO:0000269|PubMed:19805105, ECO:0000269|PubMed:20348923, ECO:0000269|PubMed:7518429}.
    分子量
    84.4 kDa Including tag.
    UniProt
    P27986
    途径
    TCR Signaling, Response to Growth Hormone Stimulus, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Hepatitis C, Protein targeting to Nucleus, VEGF Signaling, BCR Signaling, Warburg Effect
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