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PATL1 Protein (AA 1-770) (His tag)

Crystallography grade PATL1 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, Crys, SDS, WB
产品编号 ABIN3094355
发货至: 中国
  • 抗原 See all PATL1 蛋白
    PATL1 (Protein Associated with Topoisomerase II Homolog 1 (PATL1))
    蛋白类型
    Recombinant
    产品特性
    AA 1-770
    宿主
    • 1
    • 1
    资源
    • 1
    • 1
    Insect Cells
    标记
    This PATL1 protein is labelled with His tag.
    应用范围
    ELISA, Crystallization (Crys), SDS-PAGE (SDS), Western Blotting (WB)
    序列
    MFRYESLEDC PLDEDEDAFQ GLGEEDEEID QFNDDTFGSG AVDDDWQEAH ERLAELEEKL PVAVNEQTGN GERDEMDLLG DHEENLAERL SKMVIENELE DPAIMRAVQT RPVLQPQPGS LNSSIWDGSE VLRRIRGPLL AQEMPTVSVL EYALPQRPPQ GPEDDRDLSE RALPRRSTSP IIGSPPVRAV PIGTPPKQMA VPSFTQQILC PKPVHVRPPM PPRYPAPYGE RMSPNQLCSV PNSSLLGHPF PPSVPPVLSP LQRAQLLGGA QLQPGRMSPS QFARVPGFVG SPLAAMNPKL LQGRVGQMLP PAPGFRAFFS APPSATPPPQ QHPPGPGPHL QNLRSQAPMF RPDTTHLHPQ HRRLLHQRQQ QNRSQHRNLN GAGDRGSHRS SHQDHLRKDP YANLMLQREK DWVSKIQMMQ LQSTDPYLDD FYYQNYFEKL EKLSAAEEIQ GDGPKKERTK LITPQVAKLE HAYKPVQFEG SLGKLTVSSV NNPRKMIDAV VTSRSEDDET KEKQVRDKRR KTLVIIEKTY SLLLDVEDYE RRYLLSLEEE RPALMDDRKH KICSMYDNLR GKLPGQERPS DDHFVQIMCI RKGKRMVARI LPFLSTEQAA DILMTTARNL PFLIKKDAQD EVLPCLLSPF SLLLYHLPSV SITSLLRQLM NLPQSAATPA LSNPHLTAVL QNKFGLSLLL ILLSRGEDLQ SSDPATESTQ NNQWTEVMFM ATRELLRIPQ AALAKPISIP TNLVSLFSRY VDRQKLNLLE TKLQLVQGIR
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    产品特性
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human PATL1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    纯化方法
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    纯度
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    过滤
    0.22 μm filtered
    内毒素水平
    Protein is endotoxin free.
    质量等级
    Crystallography grade
  • 应用备注
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    说明

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    注意事项
    Avoid repeated freeze-thaw cycles.
    储存条件
    -80 °C
    储存方法
    Store at -80°C.
    有效期
    Unlimited (if stored properly)
  • 抗原
    PATL1 (Protein Associated with Topoisomerase II Homolog 1 (PATL1))
    别名
    PATL1 (PATL1 产品)
    别名
    Pat1b Protein, xPat1b Protein, hPat1b Protein, AV312086 Protein, D430024J04 Protein, RGD1305514 Protein, fa11d04 Protein, wu:fa11d04 Protein, zgc:158239 Protein, PAT1 homolog 1, processing body mRNA decay factor Protein, protein associated with topoisomerase II homolog 1 L homeolog Protein, protein associated with topoisomerase II homolog 1 (yeast) Protein, PATL1 Protein, patl1.L Protein, Patl1 Protein, patl1 Protein
    背景
    RNA-binding protein involved in deadenylation-dependent decapping of mRNAs, leading to the degradation of mRNAs. Acts as a scaffold protein that connects deadenylation and decapping machinery. Required for cytoplasmic mRNA processing body (P-body) assembly. In case of infection, required for translation and replication of hepatitis C virus (HCV). {ECO:0000269|PubMed:17936923, ECO:0000269|PubMed:19628699, ECO:0000269|PubMed:20543818, ECO:0000269|PubMed:20584987, ECO:0000269|PubMed:20852261}.
    分子量
    87.8 kDa Including tag.
    UniProt
    Q86TB9
    途径
    Ribonucleoprotein Complex Subunit Organization
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