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NCOA2 Protein (AA 2-1464) (His tag)

Crystallography grade NCOA2 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, Crys, SDS
产品编号 ABIN3094110
发货至: 中国
  • 抗原 See all NCOA2 蛋白
    NCOA2 (Nuclear Receptor Coactivator 2 (NCOA2))
    蛋白类型
    Recombinant
    产品特性
    AA 2-1464
    宿主
    • 2
    • 2
    • 1
    资源
    • 1
    • 1
    • 1
    Insect Cells
    标记
    This NCOA2 protein is labelled with His tag.
    应用范围
    Western Blotting (WB), ELISA, Crystallization (Crys), SDS-PAGE (SDS)
    序列
    SGMGENTSDP SRAETRKRKE CPDQLGPSPK RNTEKRNREQ ENKYIEELAE LIFANFNDID NFNFKPDKCA ILKETVKQIR QIKEQEKAAA ANIDEVQKSD VSSTGQGVID KDALGPMMLE ALDGFFFVVN LEGNVVFVSE NVTQYLRYNQ EELMNKSVYS ILHVGDHTEF VKNLLPKSIV NGGSWSGEPP RRNSHTFNCR MLVKPLPDSE EEGHDNQEAH QKYETMQCFA VSQPKSIKEE GEDLQSCLIC VARRVPMKER PVLPSSESFT TRQDLQGKIT SLDTSTMRAA MKPGWEDLVR RCIQKFHAQH EGESVSYAKR HHHEVLRQGL AFSQIYRFSL SDGTLVAAQT KSKLIRSQTT NEPQLVISLH MLHREQNVCV MNPDLTGQTM GKPLNPISSN SPAHQALCSG NPGQDMTLSS NINFPINGPK EQMGMPMGRF GGSGGMNHVS GMQATTPQGS NYALKMNSPS QSSPGMNPGQ PTSMLSPRHR MSPGVAGSPR IPPSQFSPAG SLHSPVGVCS STGNSHSYTN SSLNALQALS EGHGVSLGSS LASPDLKMGN LQNSPVNMNP PPLSKMGSLD SKDCFGLYGE PSEGTTGQAE SSCHPGEQKE TNDPNLPPAV SSERADGQSR LHDSKGQTKL LQLLTTKSDQ MEPSPLASSL SDTNKDSTGS LPGSGSTHGT SLKEKHKILH RLLQDSSSPV DLAKLTAEAT GKDLSQESSS TAPGSEVTIK QEPVSPKKKE NALLRYLLDK DDTKDIGLPE ITPKLERLDS KTDPASNTKL IAMKTEKEEM SFEPGDQPGS ELDNLEEILD DLQNSQLPQL FPDTRPGAPA GSVDKQAIIN DLMQLTAENS PVTPVGAQKT ALRISQSTFN NPRPGQLGRL LPNQNLPLDI TLQSPTGAGP FPPIRNSSPY SVIPQPGMMG NQGMIGNQGN LGNSSTGMIG NSASRPTMPS GEWAPQSSAV RVTCAATTSA MNRPVQGGMI RNPAASIPMR PSSQPGQRQT LQSQVMNIGP SELEMNMGGP QYSQQQAPPN QTAPWPESIL PIDQASFASQ NRQPFGSSPD DLLCPHPAAE SPSDEGALLD QLYLALRNFD GLEEIDRALG IPELVSQSQA VDPEQFSSQD SNIMLEQKAP VFPQQYASQA QMAQGSYSPM QDPNFHTMGQ RPSYATLRMQ PRPGLRPTGL VQNQPNQLRL QLQHRLQAQQ NRQPLMNQIS NVSNVNLTLR PGVPTQAPIN AQMLAQRQRE ILNQHLRQRQ MHQQQQVQQR TLMMRGQGLN MTPSMVAPSG MPATMSNPRI PQANAQQFPF PPNYGISQQP DPGFTGATTP QSPLMSPRMA HTQSPMMQQS QANPAYQAPS DINGWAQGNM GGNSMFSQQS PPHFGQQANT SMYSNNMNIN VSMATNTGGM SSMNQMTGQI SMTSVTSVPT SGLSSMGPEQ VNDPALRGGN LFPNQLPGMD MIKQEGDTTR KYC
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    产品特性
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human NCOA2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    纯化方法
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    纯度
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    过滤
    0.22 μm filtered
    内毒素水平
    Protein is endotoxin free.
    质量等级
    Crystallography grade
    Top Product
    Discover our top product NCOA2 蛋白
  • 应用备注
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    说明

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    注意事项
    Avoid repeated freeze-thaw cycles.
    储存条件
    -80 °C
    储存方法
    Store at -80°C.
    有效期
    Unlimited (if stored properly)
  • 抗原
    NCOA2 (Nuclear Receptor Coactivator 2 (NCOA2))
    别名
    NCOA2 (NCOA2 产品)
    别名
    GRIP1 Protein, KAT13C Protein, NCoA-2 Protein, SRC2 Protein, TIF2 Protein, bHLHe75 Protein, 9530095N19 Protein, D1Ertd433e Protein, GRIP-1 Protein, Grip1 Protein, SRC-2 Protein, TIF-2 Protein, tif2 Protein, wu:fc41a10 Protein, wu:fe26a11 Protein, Tif2 Protein, NCOA2 Protein, XTIF2 Protein, bhlhe75 Protein, grip1 Protein, kat13c Protein, src2 Protein, ncoa2-A Protein, nuclear receptor coactivator 2 Protein, nuclear receptor coactivator 2 S homeolog Protein, NCOA2 Protein, Ncoa2 Protein, ncoa2 Protein, LOC100089041 Protein, ncoa2.S Protein
    背景
    Transcriptional coactivator for steroid receptors and nuclear receptors. Coactivator of the steroid binding domain (AF-2) but not of the modulating N-terminal domain (AF-1). Required with NCOA1 to control energy balance between white and brown adipose tissues. Critical regulator of glucose metabolism regulation, acts as RORA coactivator to specifically modulate G6PC expression. Involved in the positive regulation of the transcriptional activity of the glucocorticoid receptor NR3C1 by sumoylation enhancer RWDD3. Positively regulates the circadian clock by acting as a transcriptional coactivator for the CLOCK-ARNTL/BMAL1 heterodimer (By similarity). {ECO:0000250|UniProtKB:Q61026, ECO:0000269|PubMed:23508108, ECO:0000269|PubMed:9430642}.
    分子量
    160.0 kDa Including tag.
    UniProt
    Q15596
    途径
    Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, Regulation of Lipid Metabolism by PPARalpha, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling
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