KCNMA1 Protein (AA 389-1236) (His tag)
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- 抗原 See all KCNMA1 蛋白
- KCNMA1 (Potassium Large Conductance Calcium-Activated Channel, Subfamily M, alpha Member 1 (KCNMA1))
- 蛋白类型
- Recombinant
- 产品特性
- AA 389-1236
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宿主
- 人
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资源
- Insect Cells
- 标记
- This KCNMA1 protein is labelled with His tag.
- 应用范围
- ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)
- 序列
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ELIGNRKKYG GSYSAVSGRK HIVVCGHITL ESVSNFLKDF LHKDRDDVNV EIVFLHNISP NLELEALFKR HFTQVEFYQG SVLNPHDLAR VKIESADACL ILANKYCADP DAEDASNIMR VISIKNYHPK IRIITQMLQY HNKAHLLNIP SWNWKEGDDA ICLAELKLGF IAQSCLAQGL STMLANLFSM RSFIKIEEDT WQKYYLEGVS NEMYTEYLSS AFVGLSFPTV CELCFVKLKL LMIAIEYKSA NRESRILINP GNHLKIQEGT LGFFIASDAK EVKRAFFYCK ACHDDITDPK RIKKCGCKRP KMSIYKRMRR ACCFDCGRSE RDCSCMSGRV RGNVDTLERA FPLSSVSVND CSTSFRAFED EQPSTLSPKK KQRNGGMRNS PNTSPKLMRH DPLLIPGNDQ IDNMDSNVKK YDSTGMFHWC APKEIEKVIL TRSEAAMTVL SGHVVVCIFG DVSSALIGLR NLVMPLRASN FHYHELKHIV FVGSIEYLKR EWETLHNFPK VSILPGTPLS RADLRAVNIN LCDMCVILSA NQNNIDDTSL QDKECILASL NIKSMQFDDS IGVLQANSQG FTPPGMDRSS PDNSPVHGML RQPSITTGVN IPIITELVND TNVQFLDQDD DDDPDTELYL TQPFACGTAF AVSVLDSLMS ATYFNDNILT LIRTLVTGGA TPELEALIAE ENALRGGYST PQTLANRDRC RVAQLALLDG PFADLGDGGC YGDLFCKALK TYNMLCFGIY RLRDAHLSTP SQCTKRYVIT NPPYEFELVP TDLIFCLMQF DHNAGQSRAS LSHSSHSSQS SSKKSSSVHS IPSTANRQNR PKSRESRDKQ KYVQEERL
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human KCNMA1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product KCNMA1 蛋白
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Potassium Large Conductance Calcium-Activated Channel, Subfamily M, alpha Member 1 (KCNMA1) (AA 1-1236) protein (Strep Tag)
Crystallography grade KCNMA1 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Potassium Large Conductance Calcium-Activated Channel, Subfamily M, alpha Member 1 (KCNMA1) (AA 389-1209) protein (His tag)Crystallography grade KCNMA1 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Potassium Large Conductance Calcium-Activated Channel, Subfamily M, alpha Member 1 (KCNMA1) (AA 1-1209) protein (Strep Tag)Crystallography grade KCNMA1 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- KCNMA1 (Potassium Large Conductance Calcium-Activated Channel, Subfamily M, alpha Member 1 (KCNMA1))
- 别名
- KCNMA1 (KCNMA1 产品)
- 别名
- BKTM Protein, KCa1.1 Protein, MaxiK Protein, SAKCA Protein, SLO Protein, SLO-ALPHA Protein, SLO1 Protein, bA205K10.1 Protein, mSLO1 Protein, DDBDRAFT_0190653 Protein, DDBDRAFT_0235401 Protein, DDB_0190653 Protein, DDB_0235401 Protein, KCNMA Protein, KCNMA1 Protein, 5730414M22Rik Protein, BKCa Protein, Slo Protein, Slo1 Protein, mSlo Protein, mSlo1 Protein, KCNMA1b Protein, KCNMA1c Protein, Kcnma Protein, bktm Protein, kcnma1-A Protein, sakca Protein, cSlo Protein, slo1 Protein, kcnma1 Protein, si:ch211-39f22.2 Protein, BcDNA:GH10751 Protein, CG10693 Protein, Dmel\CG10693 Protein, Dslo Protein, dSlo Protein, dSlo1 Protein, dslo Protein, fSlo Protein, potassium calcium-activated channel subfamily M alpha 1 Protein, calcium-activated BK potassium channel, alpha subunit Protein, potassium large conductance calcium-activated channel, subfamily M, alpha member 1 Protein, potassium calcium-activated channel subfamily M alpha 1 L homeolog Protein, potassium large conductance calcium-activated channel, subfamily M, alpha member 1a Protein, calcium-activated potassium channel subunit alpha-1 Protein, slowpoke Protein, KCNMA1 Protein, kcnma1 Protein, Kcnma1 Protein, kcnma1.L Protein, kcnma1a Protein, LOC100454095 Protein, slo Protein
- 背景
- Potassium channel activated by both membrane depolarization or increase in cytosolic Ca(2+) that mediates export of K(+). It is also activated by the concentration of cytosolic Mg(2+). Its activation dampens the excitatory events that elevate the cytosolic Ca(2+) concentration and/or depolarize the cell membrane. It therefore contributes to repolarization of the membrane potential. Plays a key role in controlling excitability in a number of systems, such as regulation of the contraction of smooth muscle, the tuning of hair cells in the cochlea, regulation of transmitter release, and innate immunity. In smooth muscles, its activation by high level of Ca(2+), caused by ryanodine receptors in the sarcoplasmic reticulum, regulates the membrane potential. In cochlea cells, its number and kinetic properties partly determine the characteristic frequency of each hair cell and thereby helps to establish a tonotopic map. Kinetics of KCNMA1 channels are determined by alternative splicing, phosphorylation status and its combination with modulating beta subunits. Highly sensitive to both iberiotoxin (IbTx) and charybdotoxin (CTX).
- 分子量
- 96.1 kDa Including tag.
- UniProt
- Q12791
- 途径
- Regulation of Hormone Metabolic Process, Sensory Perception of Sound
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