KCNE2 Protein (AA 1-123) (Strep Tag)
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- 抗原 See all KCNE2 蛋白
- KCNE2 (Potassium Voltage-Gated Channel, Isk-Related Family, Member 2 (KCNE2))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-123
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宿主
- 人
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资源
- Tobacco (Nicotiana tabacum)
- 标记
- This KCNE2 protein is labelled with Strep Tag.
- 应用范围
- ELISA, Western Blotting (WB), SDS-PAGE (SDS)
- 序列
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MSTLSNFTQT LEDVFRRIFI TYMDNWRQNT TAEQEALQAK VDAENFYYVI LYLMVMIGMF SFIIVAILVS TVKSKRREHS NDPYHQYIVE DWQEKYKSQI LNLEESKATI HENIGAAGFK MSP
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - 产品特性
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 内毒素水平
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product KCNE2 蛋白
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Potassium Voltage-Gated Channel, Isk-Related Family, Member 2 (KCNE2) (AA 1-123) protein (GST tag)
KCNE2 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Potassium Voltage-Gated Channel, Isk-Related Family, Member 2 (KCNE2) (AA 73-123) protein (GST tag)KCNE2 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Potassium Voltage-Gated Channel, Isk-Related Family, Member 2 (KCNE2) (AA 1-123) protein (Strep Tag)Crystallography grade KCNE2 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Potassium Voltage-Gated Channel, Isk-Related Family, Member 2 (KCNE2) (AA 1-123) protein (Strep Tag)Crystallography grade KCNE2 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Potassium Voltage-Gated Channel, Isk-Related Family, Member 2 (KCNE2) (AA 1-123), (full length) protein (His tag)KCNE2 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
Potassium Voltage-Gated Channel, Isk-Related Family, Member 2 (KCNE2) protein (Myc-DYKDDDDK Tag)KCNE2 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Potassium Voltage-Gated Channel, Isk-Related Family, Member 2 (KCNE2) (AA 70-123) protein (His tag)KCNE2 宿主: 大鼠 宿主: 酵母菌 Recombinant > 90 % ELISA
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- KCNE2 (Potassium Voltage-Gated Channel, Isk-Related Family, Member 2 (KCNE2))
- 别名
- KCNE2 (KCNE2 产品)
- 别名
- ATFB4 Protein, LQT5 Protein, LQT6 Protein, MIRP1 Protein, Mirp1 Protein, MiRP1 Protein, 2200002I16Rik Protein, AW048273 Protein, potassium voltage-gated channel subfamily E regulatory subunit 2 Protein, potassium voltage-gated channel, Isk-related subfamily, gene 2 Protein, KCNE2 Protein, Kcne2 Protein
- 背景
- Potassium voltage-gated channel subfamily E member 2 (MinK-related peptide 1) (Minimum potassium ion channel-related peptide 1) (Potassium channel subunit beta MiRP1),FUNCTION: Ancillary protein that assembles as a beta subunit with a voltage-gated potassium channel complex of pore-forming alpha subunits. Modulates the gating kinetics and enhances stability of the channel complex. Assembled with KCNB1 modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1. Associated with KCNH2/HERG is proposed to form the rapidly activating component of the delayed rectifying potassium current in heart (IKr). May associate with KCNQ2 and/or KCNQ3 and modulate the native M-type current. May associate with HCN1 and HCN2 and increase potassium current. Interacts with KCNQ1, forms a heterooligomer complex leading to currents with an apparently instantaneous activation, a rapid deactivation process and a linear current-voltage relationship and decreases the amplitude of the outward current (PubMed:11101505). KCNQ1-KCNE2 channel associates with Na(+)-coupled myo-inositol symporter in the apical membrane of choroid plexus epithelium and regulates the myo-inositol gradient between blood and cerebrospinal fluid with an impact on neuron excitability. {ECO:0000250|UniProtKB:P63161, ECO:0000250|UniProtKB:Q9D808, ECO:0000269|PubMed:10219239, ECO:0000269|PubMed:11101505}.
- 分子量
- 14.5 kDa
- UniProt
- Q9Y6J6
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