CD11c Protein (AA 20-1107) (His tag)
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- 抗原 See all CD11c (ITGAX) 蛋白
- CD11c (ITGAX) (Integrin, alpha X (Complement Component 3 Receptor 4 Subunit) (ITGAX))
- 蛋白类型
- Recombinant
- 产品特性
- AA 20-1107
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宿主
- 人
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资源
- Insect Cells
- 标记
- This CD11c protein is labelled with His tag.
- 应用范围
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
- 序列
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FNLDTEELTA FRVDSAGFGD SVVQYANSWV VVGAPQKITA ANQTGGLYQC GYSTGACEPI GLQVPPEAVN MSLGLSLAST TSPSQLLACG PTVHHECGRN MYLTGLCFLL GPTQLTQRLP VSRQECPRQE QDIVFLIDGS GSISSRNFAT MMNFVRAVIS QFQRPSTQFS LMQFSNKFQT HFTFEEFRRS SNPLSLLASV HQLQGFTYTA TAIQNVVHRL FHASYGARRD AAKILIVITD GKKEGDSLDY KDVIPMADAA GIIRYAIGVG LAFQNRNSWK ELNDIASKPS QEHIFKVEDF DALKDIQNQL KEKIFAIEGT ETTSSSSFEL EMAQEGFSAV FTPDGPVLGA VGSFTWSGGA FLYPPNMSPT FINMSQENVD MRDSYLGYST ELALWKGVQS LVLGAPRYQH TGKAVIFTQV SRQWRMKAEV TGTQIGSYFG ASLCSVDVDS DGSTDLVLIG APHYYEQTRG GQVSVCPLPR GWRRWWCDAV LYGEQGHPWG RFGAALTVLG DVNGDKLTDV VIGAPGEEEN RGAVYLFHGV LGPSISPSHS QRIAGSQLSS RLQYFGQALS GGQDLTQDGL VDLAVGARGQ VLLLRTRPVL WVGVSMQFIP AEIPRSAFEC REQVVSEQTL VQSNICLYID KRSKNLLGSR DLQSSVTLDL ALDPGRLSPR ATFQETKNRS LSRVRVLGLK AHCENFNLLL PSCVEDSVTP ITLRLNFTLV GKPLLAFRNL RPMLAADAQR YFTASLPFEK NCGADHICQD NLGISFSFPG LKSLLVGSNL ELNAEVMVWN DGEDSYGTTI TFSHPAGLSY RYVAEGQKQG QLRSLHLTCD SAPVGSQGTW STSCRINHLI FRGGAQITFL ATFDVSPKAV LGDRLLLTAN VSSENNTPRT SKTTFQLELP VKYAVYTVVS SHEQFTKYLN FSESEEKESH VAMHRYQVNN LGQRDLPVSI NFWVPVELNQ EAVWMDVEVS HPQNPSLRCS SEKIAPPASD FLAHIQKNPV LDCSIAGCLR FRCDVPSFSV QEELDFTLKG NLSFGWVRQI LQKKVSVVSV AEITFDTSVY SQLPGQEAFM RAQTTTVLEK YKVHNPTP
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human ITGAX Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product ITGAX 蛋白
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Integrin, alpha X (Complement Component 3 Receptor 4 Subunit) (ITGAX) (AA 1124-1163) protein (His-IF2DI Tag)
ITGAX 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Integrin, alpha X (Complement Component 3 Receptor 4 Subunit) (ITGAX) (AA 114-223) protein (GST tag)ITGAX 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Integrin, alpha X (Complement Component 3 Receptor 4 Subunit) (ITGAX) (AA 20-1116) protein (His tag)Crystallography grade ITGAX 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Integrin, alpha X (Complement Component 3 Receptor 4 Subunit) (ITGAX) (AA 20-1163) protein (rho-1D4 tag)Crystallography grade ITGAX 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Integrin, alpha X (Complement Component 3 Receptor 4 Subunit) (ITGAX) (AA 20-1169) protein (rho-1D4 tag)Crystallography grade ITGAX 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- CD11c (ITGAX) (Integrin, alpha X (Complement Component 3 Receptor 4 Subunit) (ITGAX))
- 别名
- ITGAX (ITGAX 产品)
- 别名
- CD11C Protein, SLEB6 Protein, RGD1561123 Protein, AI449405 Protein, Cd11c Protein, Cr4 Protein, N418 Protein, integrin subunit alpha X Protein, integrin alpha X Protein, ITGAX Protein, Itgax Protein
- 背景
- Integrin alpha-X/beta-2 is a receptor for fibrinogen. It recognizes the sequence G-P-R in fibrinogen. It mediates cell-cell interaction during inflammatory responses. It is especially important in monocyte adhesion and chemotaxis.
- 分子量
- 120.8 kDa Including tag.
- UniProt
- P20702
- 途径
- Complement System, Activated T Cell Proliferation, Integrin Complex
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