GLDN Protein (AA 95-454) (rho-1D4 tag)
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- 抗原 See all GLDN 蛋白
- GLDN (Gliomedin (GLDN))
- 蛋白类型
- Recombinant
- 产品特性
- AA 95-454
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宿主
- 人
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资源
- Insect Cells
- 标记
- This GLDN protein is labelled with rho-1D4 tag.
- 应用范围
- ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)
- 序列
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SHSGEPAPHI RAESHDMLMM MTYSMVPIRV MVDLCNSTKG ICLTGPSGPP GPPGAGGLPG HNGLDGQPGP QGPKGEKGAN GKRGKMGIPG AAGNPGERGE KGDHGELGLQ GNEGPPGQKG EKGDKGDVSN DVLLAGAKGD QGPPGPPGPP GPPGPPGPPG SRRAKGPRQP SMFNGQCPGE TCAIPNDDTL VGKADEKASE HHSPQAESMI TSIGNPVQVL KVTETFGTWI RESANKSDDR IWVTEHFSGI MVKEFKDQPS LLNGSYTFIH LPYYFHGCGH VVYNNSLYYH KGGSNTLVRF EFGQETSQTL KLENALYFDR KYLFANSKTY FNLAVDEKGL WIIYASSVDG SSILVAQLDE
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human GLDN Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
- Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
- The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin-free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product GLDN 蛋白
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Gliomedin (GLDN) (AA 131-240) protein (GST tag)
GLDN 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Gliomedin (GLDN) (AA 1-551) protein (Strep Tag)Crystallography grade GLDN 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Gliomedin (GLDN) (AA 40-551) protein (His tag)Crystallography grade GLDN 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Gliomedin (GLDN) (AA 39-549) protein (His tag)Crystallography grade GLDN 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Gliomedin (GLDN) (AA 95-454) protein (rho-1D4 tag)Crystallography grade GLDN 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Gliomedin (GLDN) (AA 1-549) protein (Strep Tag)Crystallography grade GLDN 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Gliomedin (GLDN) (AA 95-451) protein (rho-1D4 tag)Crystallography grade GLDN 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Gliomedin (GLDN) protein (Myc-DYKDDDDK Tag)GLDN 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- GLDN (Gliomedin (GLDN))
- 别名
- GLDN (GLDN 产品)
- 别名
- CLOM Protein, COLM Protein, CRG-L2 Protein, CRGL2 Protein, UNC-112 Protein, Clom Protein, Colm Protein, Crgl2 Protein, Crlg2 Protein, gliomedin Protein, GLDN Protein, Gldn Protein, gldn Protein
- 背景
- Ligand for NRCAM and NFASC/neurofascin that plays a role in the formation and maintenance of the nodes of Ranvier on myelinated axons. Mediates interaction between Schwann cell microvilli and axons via its interactions with NRCAM and NFASC. Nodes of Ranvier contain clustered sodium channels that are crucial for the saltatory propagation of action potentials along myelinated axons. During development, nodes of Ranvier are formed by the fusion of two heminodes. Required for normal clustering of sodium channels at heminodes, not required for the formation of mature nodes with normal sodium channel clusters. Required, together with NRCAM, for maintaining NFASC and sodium channel clusters at mature nodes of Ranvier. {ECO:0000250|UniProtKB:Q8BMF8}.
- 分子量
- 39.3 kDa Including tag.
- UniProt
- Q6ZMI3
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