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COPS4 Protein (AA 2-406) (His tag)

Crystallography grade COPS4 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB, Crys
产品编号 ABIN3092016
发货至: 中国
  • 抗原 See all COPS4 蛋白
    COPS4 (COP9 Signalosome Subunit 4 (COPS4))
    蛋白类型
    Recombinant
    产品特性
    AA 2-406
    宿主
    • 1
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    资源
    • 8
    • 1
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    Insect Cells
    标记
    This COPS4 protein is labelled with His tag.
    应用范围
    ELISA, SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys)
    序列
    AAAVRQDLAQ LMNSSGSHKD LAGKYRQILE KAIQLSGAEQ LEALKAFVEA MVNENVSLVI SRQLLTDFCT HLPNLPDSTA KEIYHFTLEK IQPRVISFEE QVASIRQHLA SIYEKEEDWR NAAQVLVGIP LETGQKQYNV DYKLETYLKI ARLYLEDDDP VQAEAYINRA SLLQNESTNE QLQIHYKVCY ARVLDYRRKF IEAAQRYNEL SYKTIVHESE RLEALKHALH CTILASAGQQ RSRMLATLFK DERCQQLAAY GILEKMYLDR IIRGNQLQEF AAMLMPHQKA TTADGSSILD RAVIEHNLLS ASKLYNNITF EELGALLEIP AAKAEKIASQ MITEGRMNGF IDQIDGIVHF ETREALPTWD KQIQSLCFQV NNLLEKISQT APEWTAQAME AQMAQ
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    产品特性
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human COPS4 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    纯化方法
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    纯度
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    过滤
    0.22 μm filtered
    内毒素水平
    Protein is endotoxin free.
    质量等级
    Crystallography grade
  • 应用备注
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    说明

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    注意事项
    Avoid repeated freeze-thaw cycles.
    储存条件
    -80 °C
    储存方法
    Store at -80°C.
    有效期
    Unlimited (if stored properly)
  • 抗原
    COPS4 (COP9 Signalosome Subunit 4 (COPS4))
    别名
    COPS4 (COPS4 产品)
    别名
    CG8725 Protein, CH4 Protein, Cops4 Protein, Csn4 Protein, DCH4 Protein, Dch4 Protein, Dmel\\CG8725 Protein, csn4 Protein, l(2)k08018 Protein, COPS4 Protein, SGN4 Protein, DKFZp459H0324 Protein, AW208976 Protein, D5Ertd774e Protein, fc90c08 Protein, wu:fc90c08 Protein, zgc:77137 Protein, ATS4 Protein, CONSTITUTIVE PHOTOMORPHOGENIC 14 Protein, CONSTITUTIVE PHOTOMORPHOGENIC 8 Protein, COP14 Protein, COP9 SIGNALOSOME SUBUNIT 4 Protein, CSN4 Protein, EMB134 Protein, EMBRYO DEFECTIVE 134 Protein, FUS4 Protein, FUS8 Protein, FUSCA 4 Protein, FUSCA 8 Protein, MBD2.17 Protein, MBD2_17 Protein, COS41.8 Protein, COP9 signalosome subunit 4 Protein, COP9 signalosome complex subunit 4 Protein, COP9 constitutive photomorphogenic homolog subunit 4 (Arabidopsis) Protein, Proteasome component (PCI) domain protein Protein, COP9 signalosome subunit 4 L homeolog Protein, CSN4 Protein, COPS4 Protein, CpipJ_CPIJ000966 Protein, csn4 Protein, Cops4 Protein, cops4 Protein, COP8 Protein, cops4.L Protein, csn-4 Protein
    背景
    Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. Also involved in the deneddylation of non-cullin subunits such as STON2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1, IRF8/ICSBP and SNAPIN, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. {ECO:0000269|PubMed:11285227, ECO:0000269|PubMed:11337588, ECO:0000269|PubMed:12628923, ECO:0000269|PubMed:12732143, ECO:0000269|PubMed:21102408, ECO:0000269|PubMed:9535219}.
    分子量
    47.1 kDa Including tag.
    UniProt
    Q9BT78
    途径
    Cell Division Cycle
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