OB Cadherin Protein (AA 54-617) (MBP tag)
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- 抗原 See all OB Cadherin (CDH11) 蛋白
- OB Cadherin (CDH11) (Cadherin 11 (CDH11))
- 蛋白类型
- Recombinant
- 产品特性
- AA 54-617
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宿主
- 人
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资源
- Insect Cells
- 标记
- This OB Cadherin protein is labelled with MBP tag.
- 应用范围
- SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)
- 序列
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GWVWNQFFVI EEYTGPDPVL VGRLHSDIDS GDGNIKYILS GEGAGTIFVI DDKSGNIHAT KTLDREERAQ YTLMAQAVDR DTNRPLEPPS EFIVKVQDIN DNPPEFLHET YHANVPERSN VGTSVIQVTA SDADDPTYGN SAKLVYSILE GQPYFSVEAQ TGIIRTALPN MDREAKEEYH VVIQAKDMGG HMGGLSGTTK VTITLTDVND NPPKFPQSVY QMSVSEAAVP GEEVGRVKAK DPDIGENGLV TYNIVDGDGM ESFEITTDYE TQEGVIKLKK PVDFETKRAY SLKVEAANVH IDPKFISNGP FKDTVTVKIS VEDADEPPMF LAPSYIHEVQ ENAAAGTVVG RVHAKDPDAA NSPIRYSIDR HTDLDRFFTI NPEDGFIKTT KPLDREETAW LNITVFAAEI HNRHQEAKVP VAIRVLDVND NAPKFAAPYE GFICESDQTK PLSNQPIVTI SADDKDDTAN GPRFIFSLPP EIIHNPNFTV RDNRDNTAGV YARRGGFSRQ KQDLYLLPIV ISDGGIPPMS STNTLTIKVC GCDVNGALLS CNAEAYILNA GLST
Sequence without tag. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human CDH11 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
- The protein is purified from the cleared cell lysate using MBP capture materials. Eluate fractions are analyzed by SDS-PAGE. Protein containing fractions are subjected to a second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin-free.
- 质量等级
- Crystallography grade
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Cadherin 11 (CDH11) (AA 54-617) protein (His tag)
CDH11 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Tris-Bis PAGE
Cadherin 11 (CDH11) (AA 509-617) protein (GST tag)CDH11 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Cadherin 11 (CDH11) (AA 54-617) protein (His tag)CDH11 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Cadherin 11 (CDH11) (AA 54-617) protein (His-DYKDDDDK-Strep II Tag)CDH11 宿主: 人 宿主: HEK-293T Cells Recombinant SDS, WB, ELISA, PI
Cadherin 11 (CDH11) (AA 641-796) protein (His tag)Crystallography grade CDH11 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Cadherin 11 (CDH11) (AA 54-617) protein (His tag)Crystallography grade CDH11 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Cadherin 11 (CDH11) (AA 641-796) protein (His tag)Crystallography grade CDH11 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Cadherin 11 (CDH11) (AA 54-796) protein (rho-1D4 tag)Crystallography grade CDH11 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Cadherin 11 (CDH11) (AA 54-796) protein (rho-1D4 tag)Crystallography grade CDH11 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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N-terminal MBP-Tag
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- in solution (20 mM Hepes, pH 7.4, 5 mM NaCl)
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- OB Cadherin (CDH11) (Cadherin 11 (CDH11))
- 别名
- CDH11 (CDH11 产品)
- 别名
- cad11 Protein, cdhob Protein, osf-4 Protein, Xcad-11 Protein, MGC108014 Protein, OB-cadherin Protein, cadherin-11 Protein, Cad11 Protein, cdhvn Protein, vnc Protein, CAD11 Protein, CDHOB Protein, OB Protein, OSF-4 Protein, cadherin 11 Protein, cadherin 11, type 2, OB-cadherin (osteoblast) Protein, cadherin 11 S homeolog Protein, CDH11 Protein, cdh11 Protein, Cdh11 Protein, cdh11.S Protein
- 背景
- Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells, cadherins may thus contribute to the sorting of heterogeneous cell types.
- 分子量
- 62.9 kDa Including tag.
- UniProt
- P55287
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