CDH18 Protein (AA 54-608) (MBP tag)
-
- 抗原 See all CDH18 蛋白
- CDH18 (Cadherin 18, Type 2 (CDH18))
- 蛋白类型
- Recombinant
- 产品特性
- AA 54-608
- 宿主
- 人
-
资源
- Insect Cells
- 标记
- This CDH18 protein is labelled with MBP tag.
- 应用范围
- Western Blotting (WB), ELISA, SDS-PAGE (SDS), Crystallization (Crys)
- 序列
-
GWVWNQFFVL EEHMGPDPQY VGKLHSNSDK GDGSVKYILT GEGAGTIFII DDTTGDIHST KSLDREQKTH YVLHAQAIDR RTNKPLEPES EFIIKVQDIN DNAPKFTDGP YIVTVPEMSD MGTSVLQVTA TDADDPTYGN SARVVYSILQ GQPYFSVDPK TGVIRTALHN MDREAREHYS VVIQAKDMAG QVGGLSGSTT VNITLTDVND NPPRFPQKHY QLYVPESAQV GSAVGKIKAN DADTGSNADM TYSIINGDGM GIFSISTDKE TREGILSLKK PLNYEKKKSY TLNIEGANTH LDFRFSHLGP FKDATMLKII VGDVDEPPLF SMPSYLMEVY ENAKIGTVVG TVLAQDPDST NSLVRYFINY NVEDDRFFNI DANTGTIRTT KVLDREETPW YNITVTASEI DNPDLLSHVT VGIRVLDVND NPPELAREYD IIVCENSKPG QVIHTISATD KDDFANGPRF NFFLDERLPV NPNFTLKDNE DNTASILTRR RRFSRTVQDV YYLPIMISDG GIPSLSSSST LTIRVCACER DGRVRTCHAE AFLSS
Sequence without tag. - 产品特性
-
- Made in Germany - from design to production - by highly experienced protein experts.
- Human CDH18 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
- The protein is purified from the cleared cell lysate using MBP capture materials. Eluate fractions are analyzed by SDS-PAGE. Protein containing fractions are subjected to a second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin-free.
- 质量等级
- Crystallography grade
-
Cadherin 18, Type 2 (CDH18) (AA 1-790) protein (GST tag)
CDH18 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Cadherin 18, Type 2 (CDH18) (AA 467-576) protein (GST tag)CDH18 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Cadherin 18, Type 2 (CDH18) (AA 54-608) protein (His tag)CDH18 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Cadherin 18, Type 2 (CDH18) (AA 637-790) protein (His tag)Crystallography grade CDH18 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS, Crys
Cadherin 18, Type 2 (CDH18) (AA 54-790) protein (rho-1D4 tag)Crystallography grade CDH18 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS, Crys
-
- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
-
N-terminal MBP-Tag
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 缓冲液
- in solution (20 mM Hepes, pH 7.4, 5 mM NaCl)
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
-
- 抗原
- CDH18 (Cadherin 18, Type 2 (CDH18))
- 别名
- CDH18 (CDH18 产品)
- 别名
- B230220E17Rik Protein, Cdh14l Protein, CDH14 Protein, CDH14L Protein, CDH24 Protein, cadherin 18 Protein, Cdh18 Protein, CDH18 Protein
- 背景
- Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells, cadherins may thus contribute to the sorting of heterogeneous cell types.
- 分子量
- 62.6 kDa Including tag.
- UniProt
- Q13634
- 途径
- Cell-Cell Junction Organization
-
