电话:
400-7060-959
传真:
+86 10 56315212-8813
电子邮件:
orders@antibodies-online.cn

Cadherin 8 Protein (CDH8) (AA 62-621) (MBP tag)

Crystallography grade CDH8 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
产品编号 ABIN3090232
发货至: 中国
  • 抗原 See all Cadherin 8 (CDH8) 蛋白
    Cadherin 8 (CDH8)
    蛋白类型
    Recombinant
    产品特性
    AA 62-621
    宿主
    • 7
    • 3
    • 3
    资源
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    Insect Cells
    标记
    This Cadherin 8 protein is labelled with MBP tag.
    应用范围
    ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)
    序列
    M K T E E G K L V I W I N G D K G Y N G L A E V G K K F E K D T G I K V T V E H P D K L E E K F P Q V A A T G D G P D I I F W A H D R F G G Y A Q S G L L A E I T P D K A F Q D K L Y P F T W D A V R Y N G K L I A Y P I A V E A L S L I Y N K D L L P N P P K T W E E I P A L D K E L K A K G K S A L M F N L Q E P Y F T W P L I A A D G G Y A F K Y E N G K Y D I K D V G V D N A G A K A G L T F L V D L I K N K H M N A D T D Y S I A E A A F N K G E T A M T I N G P W A W S N I D T S K V N Y G V T V L P T F K G Q P S K P F V G V L S A G I N A A S P N K E L A K E F L E N Y L L T D E G L E A V N K D K P L G A V A L K S Y E E E L A K D P R I A A T M E N A Q K G E I M P N I P Q M S A F W Y A V R T A V I N A A S G R Q T V D E A L K D A Q T G G G S G G G S E N L Y F Q S A A A
    GWVWNQMFVL EEFSGPEPIL VGRLHTDLDP GSKKIKYILS GDGAGTIFQI NDVTGDIHAI KRLDREEKAE YTLTAQAVDW ETSKPLEPPS EFIIKVQDIN DNAPEFLNGP YHATVPEMSI LGTSVTNVTA TDADDPVYGN SAKLVYSILE GQPYFSIEPE TAIIKTALPN MDREAKEEYL VVIQAKDMGG HSGGLSGTTT LTVTLTDVND NPPKFAQSLY HFSVPEDVVL GTAIGRVKAN DQDIGENAQS SYDIIDGDGT ALFEITSDAQ AQDGIIRLRK PLDFETKKSY TLKVEAANVH IDPRFSGRGP FKDTATVKIV VEDADEPPVF SSPTYLLEVH ENAALNSVIG QVTARDPDIT SSPIRFSIDR HTDLERQFNI NADDGKITLA TPLDRELSVW HNITIIATEI RNHSQISRVP VAIKVLDVND NAPEFASEYE AFLCENGKPG QVIQTVSAMD KDDPKNGHYF LYSLLPEMVN NPNFTIKKNE DNSLSILAKH NGFNRQKQEV YLLPIIISDS GNPPLSSTST LTIRVCGCSN DGVVQSCNVE AYVLPIGLSM
    Sequence with tag.
    产品特性
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human CDH8 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    纯化方法
    The protein is purified from the cleared cell lysate using MBP capture materials. Eluate fractions are analyzed by SDS-PAGE. Protein containing fractions are subjected to a second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    纯度
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    过滤
    0.22 μm filtered
    内毒素水平
    Protein is endotoxin-free.
    质量等级
    Crystallography grade
  • 应用备注
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    说明

    N-terminal MBP-Tag

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    20 mM Hepes, pH 7.4, 5 mM NaCl
    注意事项
    Avoid repeated freeze-thaw cycles.
    储存条件
    -80 °C
    储存方法
    Store at -80°C.
    有效期
    Unlimited (if stored properly)
  • 抗原
    Cadherin 8 (CDH8)
    别名
    CDH8 (CDH8 产品)
    别名
    AI851472 Protein, cad8 Protein, cadherin-8 Protein, Nbla04261 Protein, cadherin 8 Protein, cadherin 8, type 2 Protein, Cdh8 Protein, CDH8 Protein
    背景
    Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells, cadherins may thus contribute to the sorting of heterogeneous cell types.
    分子量
    62.4 kDa Including tag.
    UniProt
    P55286
    途径
    Cell-Cell Junction Organization
You are here:
客服