BAG1 Protein (AA 1-345) (Strep Tag)
Quick Overview for BAG1 Protein (AA 1-345) (Strep Tag) (ABIN3089747)
抗原
See all BAG1 蛋白蛋白类型
宿主
资源
应用范围
纯度
质量等级
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产品特性
- AA 1-345
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标记
- This BAG1 protein is labelled with Strep Tag.
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序列
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MAQRGGARRP RGDRERLGSR LRALRPGREP RQSEPPAQRG PPPSGRPPAR STASGHDRPT RGAAAGARRP RMKKKTRRRS TRSEELTRSE ELTLSEEATW SEEATQSEEA TQGEEMNRSQ EVTRDEESTR SEEVTREEMA AAGLTVTVTH SNEKHDLHVT SQQGSSEPVV QDLAQVVEEV IGVPQSFQKL IFKGKSLKEM ETPLSALGIQ DGCRVMLIGK KNSPQEEVEL KKLKHLEKSV EKIADQLEEL NKELTGIQQG FLPKDLQAEA LCKLDRRVKA TIEQFMKILE EIDTLILPEN FKDSRLKRKG LVKKVQAFLA ECDTVEQNIC QETERLQSTN FALAE
Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.. -
产品特性
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified in one-step affinity chromatography
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a predefined custom protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.
The big advantage of ordering our predefined custom proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
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纯化方法
- One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®).
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想要此Protein的其他选项吗？
!探索我们的目录蛋白和定制蛋白服务选项！Product表达系统ConjugateOriginPrice表达系统 Wheat germConjugate GST tagOrigin HumanPrice 5,859.06 ¥表达系统 Wheat germConjugate GST tagOrigin HumanPrice 5,859.06 ¥表达系统 Escherichia coli (E. coli)ConjugateOrigin HumanPrice 15,975.36 ¥表达系统 Escherichia coli (E. coli)Conjugate His tagOrigin HumanPrice 3,090.68 ¥表达系统 Escherichia coli (E. coli)Conjugate GST tagOrigin HumanPrice 3,725.09 ¥您的项目需要进一步定制吗？联系我们，了解我们的定制蛋白解决方案
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BCL2-Associated Athanogene (BAG1) (AA 1-355) protein (His tag)
custom-made BAG1 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
BCL2-Associated Athanogene (BAG1) (AA 1-345) protein (His tag)custom-made BAG1 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
BCL2-Associated Athanogene (BAG1) (AA 241-345) protein (GST tag)BAG1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
BCL2-Associated Athanogene (BAG1) (AA 1-274) protein (GST tag)BAG1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
BCL2-Associated Athanogene (BAG1) (AA 1-230) proteinBAG1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % pure SDS
BCL2-Associated Athanogene (BAG1) proteinBAG1 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant WB, SDS, ELISA, IP
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应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
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说明
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! -
限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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The buffer composition is at the discretion of the manufacturer.
Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein. -
注意事项
- Avoid repeated freeze-thaw cycles.
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储存条件
- -80 °C
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储存方法
- Store at -80°C.
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有效期
- 12 months
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- BAG1 (BCL2-Associated Athanogene (BAG1))
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别名
- BAG1
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背景
- BAG family molecular chaperone regulator 1 (BAG-1) (Bcl-2-associated athanogene 1),FUNCTION: Co-chaperone for HSP70 and HSC70 chaperone proteins. Acts as a nucleotide-exchange factor (NEF) promoting the release of ADP from the HSP70 and HSC70 proteins thereby triggering client/substrate protein release. Nucleotide release is mediated via its binding to the nucleotide-binding domain (NBD) of HSPA8/HSC70 where as the substrate release is mediated via its binding to the substrate-binding domain (SBD) of HSPA8/HSC70 (PubMed:27474739, PubMed:9873016, PubMed:24318877). Inhibits the pro-apoptotic function of PPP1R15A, and has anti-apoptotic activity (PubMed:12724406). Markedly increases the anti-cell death function of BCL2 induced by various stimuli (PubMed:9305631). Involved in the STUB1-mediated proteasomal degradation of ESR1 in response to age-related circulating estradiol (17-beta-estradiol/E2) decline, thereby promotes neuronal apoptosis in response to ischemic reperfusion injury (By similarity). {ECO:0000250|UniProtKB:B0K019, ECO:0000269|PubMed:12724406, ECO:0000269|PubMed:24318877, ECO:0000269|PubMed:27474739, ECO:0000269|PubMed:9305631, ECO:0000269|PubMed:9873016}.
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分子量
- 38.8 kDa
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UniProt
- Q99933
抗原
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