ATP2C1 Protein (AA 313-699) (His tag)
(1 reference)-
- 抗原 See all ATP2C1 蛋白
- ATP2C1 (ATPase, Ca++ Transporting, Type 2C, Member 1 (ATP2C1))
- 蛋白类型
- Recombinant
- 产品特性
- AA 313-699
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This ATP2C1 protein is labelled with His tag.
- 应用范围
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
- 序列
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MVVTVTLALG VMRMVKKRAI VKKLPIVETL GCCNVICSDK TGTLTKNEMT VTHIFTSDGL H AEVTGVGYNQ FGEVIVDGDV VHGFYNPAVS RIVEAGCVCN DAVIRNNTLM GKPTEGALIA LAMKMGLDGL QQDYIRKAEY PFSSEQKWMA VKCVHRTQQD RPEICFMKGA YEQVIKYCTT YQSKGQTLTL TQQQRDVYQQ EKARMGSAGL RVLALASGPE LGQLTFLGLV GIIDPPRTGV KEAVTTLIAS GVSIKMITGD SQETAVAIAS RLGLYSKTSQ SVSGEEIDAM DVQQLSQIVP KVAVFYRASP RHKMKIIKSL QKNGSVVAMT GDGVN
Sequence includes C-terminal His-tag - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human PLP1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
- The protein is purified from the cleared cell lysate using His-tag capture materials. Eluate fractions are analyzed by SDS-PAGE. Protein containing fractions are subjected to a second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin free.
- 质量等级
- Crystallography grade
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ATPase, Ca++ Transporting, Type 2C, Member 1 (ATP2C1) (AA 119-269) protein (GST tag)
ATP2C1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
ATPase, Ca++ Transporting, Type 2C, Member 1 (ATP2C1) (AA 1-919) protein (Strep Tag)Crystallography grade ATP2C1 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
ATPase, Ca++ Transporting, Type 2C, Member 1 (ATP2C1) (AA 124-262) protein (His tag)Crystallography grade ATP2C1 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
ATPase, Ca++ Transporting, Type 2C, Member 1 (ATP2C1) (AA 122-262) protein (His tag)Crystallography grade ATP2C1 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
ATPase, Ca++ Transporting, Type 2C, Member 1 (ATP2C1) (AA 317-699) protein (His tag)Crystallography grade ATP2C1 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
ATPase, Ca++ Transporting, Type 2C, Member 1 (ATP2C1) (AA 1-918) protein (Strep Tag)Crystallography grade ATP2C1 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- In solution (30 mM Hepes, pH 8.0, 100 mM NaCl, Laurylsarcosine)
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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: "Synergy among non-desmoglein antibodies contributes to the immunopathology of desmoglein antibody-negative pemphigus vulgaris." in: The Journal of biological chemistry, Vol. 294, Issue 12, pp. 4520-4528, (2019) (PubMed).
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: "Synergy among non-desmoglein antibodies contributes to the immunopathology of desmoglein antibody-negative pemphigus vulgaris." in: The Journal of biological chemistry, Vol. 294, Issue 12, pp. 4520-4528, (2019) (PubMed).
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- 抗原
- ATP2C1 (ATPase, Ca++ Transporting, Type 2C, Member 1 (ATP2C1))
- 别名
- ATP2C1 (ATP2C1 产品)
- 别名
- ATP2C1 Protein, ATP2C1A Protein, BCPM Protein, HHD Protein, PMR1 Protein, SPCA1 Protein, hSPCA1 Protein, Spca1 Protein, si:dkey-11p23.6 Protein, SPCA Protein, 1700121J11Rik Protein, AW061228 Protein, D930003G21Rik Protein, pmr1 Protein, ATPase secretory pathway Ca2+ transporting 1 Protein, ATPase, Ca++ transporting, type 2C, member 1 Protein, ATPase, Ca++-sequestering Protein, ATP2C1 Protein, atp2c1 Protein, Atp2c1 Protein
- 背景
- This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of the calcium.
- 分子量
- 42.7 kDa including tag.
- UniProt
- P98194
- 途径
- Transition Metal Ion Homeostasis, Ribonucleoside Biosynthetic Process
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