ARHGEF7 Protein (AA 1-803) (Strep Tag)
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- 抗原 See all ARHGEF7 蛋白
- ARHGEF7 (rho Guanine Nucleotide Exchange Factor (GEF) 7 (ARHGEF7))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-803
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宿主
- 人
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资源
- Tobacco (Nicotiana tabacum)
- 标记
- This ARHGEF7 protein is labelled with Strep Tag.
- 应用范围
- Western Blotting (WB), SDS-PAGE (SDS), ELISA
- 序列
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MNSAEQTVTW LITLGVLESP KKTISDPEGF LQASLKDGVV LCRLLERLLP GTIEKVYPEP RSESECLSNI REFLRGCGAS LRLELLFPPS QPPQHLVTTI LLSASTFDAN DLYQGQNFNK VLSSLVTLNK VTADIGLGSD SVCARPSSHR IKSFDSLGSQ SLHTRTSKLF QGQYRSLDMT DNSNNQLVVR AKFNFQQTNE DELSFSKGDV IHVTRVEEGG WWEGTLNGRT GWFPSNYVRE VKASEKPVSP KSGTLKSPPK GFDTTAINKS YYNVVLQNIL ETENEYSKEL QTVLSTYLRP LQTSEKLSSA NISYLMGNLE EICSFQQMLV QSLEECTKLP EAQQRVGGCF LNLMPQMKTL YLTYCANHPS AVNVLTEHSE ELGEFMETKG ASSPGILVLT TGLSKPFMRL DKYPTLLKEL ERHMEDYHTD RQDIQKSMAA FKNLSAQCQE VRKRKELELQ ILTEAIRNWE GDDIKTLGNV TYMSQVLIQC AGSEEKNERY LLLFPNVLLM LSASPRMSGF IYQGKLPTTG MTITKLEDSE NHRNAFEISG SMIERILVSC NNQQDLQEWV EHLQKQTKVT SVGNPTIKPH SVPSHTLPSH PVTPSSKHAD SKPAPLTPAY HTLPHPSHHG TPHTTINWGP LEPPKTPKPW SLSCLRPAPP LRPSAALCYK EDLSKSPKTM KKLLPKRKPE RKPSDEEFAS RKSTAALEED AQILKVIEAY CTSAKTRQTL NSTWQGTDLM HNHVLADDDQ PSLDSLGRRS SLSRLEPSDL SEDSDYDSIW TAHSYRMGST SRKSCCSYIS HQN
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - 产品特性
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 内毒素水平
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product ARHGEF7 蛋白
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rho Guanine Nucleotide Exchange Factor (GEF) 7 (ARHGEF7) (AA 264-450) protein (His tag)
ARHGEF7 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
rho Guanine Nucleotide Exchange Factor (GEF) 7 (ARHGEF7) (AA 102-190) protein (GST tag)ARHGEF7 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
rho Guanine Nucleotide Exchange Factor (GEF) 7 (ARHGEF7) (AA 1-862) protein (Strep Tag)Crystallography grade ARHGEF7 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
rho Guanine Nucleotide Exchange Factor (GEF) 7 (ARHGEF7) (AA 1-250), (partial) protein (GST tag)ARHGEF7 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
rho Guanine Nucleotide Exchange Factor (GEF) 7 (ARHGEF7) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)ARHGEF7 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
rho Guanine Nucleotide Exchange Factor (GEF) 7 (ARHGEF7) (Transcript Variant 5) protein (Myc-DYKDDDDK Tag)ARHGEF7 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- ARHGEF7 (rho Guanine Nucleotide Exchange Factor (GEF) 7 (ARHGEF7))
- 别名
- ARHGEF7 (ARHGEF7 产品)
- 别名
- BETA-PIX Protein, COOL-1 Protein, COOL1 Protein, Nbla10314 Protein, P50 Protein, P50BP Protein, P85 Protein, P85COOL1 Protein, P85SPR Protein, PAK3 Protein, PIXB Protein, cool1 Protein, p50 Protein, p85 Protein, pak3 Protein, pixb Protein, ARHGEF7 Protein, P85spr Protein, Pak3bp Protein, Cool Protein, PIX Protein, betaPix Protein, betaPix-b Protein, betaPix-c Protein, cool-1 Protein, mKIAA0142 Protein, p85Cool1 Protein, p85SPR Protein, arhgef7 Protein, bbh Protein, bpix Protein, im:7142367 Protein, wu:fc35h08 Protein, zgc:101775 Protein, Rho guanine nucleotide exchange factor 7 Protein, Rho guanine nucleotide exchange factor 7 S homeolog Protein, ankyrin repeat domain 10 Protein, Rho guanine nucleotide exchange factor (GEF7) Protein, Rho guanine nucleotide exchange factor (GEF) 7b Protein, ARHGEF7 Protein, arhgef7.S Protein, arhgef7 Protein, ANKRD10 Protein, Arhgef7 Protein, arhgef7b Protein
- 背景
- Rho guanine nucleotide exchange factor 7 (Beta-Pix) (COOL-1) (PAK-interacting exchange factor beta) (p85),FUNCTION: Acts as a RAC1 guanine nucleotide exchange factor (GEF) and can induce membrane ruffling. Functions in cell migration, attachment and cell spreading. Promotes targeting of RAC1 to focal adhesions (By similarity). May function as a positive regulator of apoptosis. Downstream of NMDA receptors and CaMKK-CaMK1 signaling cascade, promotes the formation of spines and synapses in hippocampal neurons. {ECO:0000250, ECO:0000269|PubMed:18184567, ECO:0000269|PubMed:18716323, ECO:0000269|PubMed:19041750}.
- 分子量
- 90.0 kDa
- UniProt
- Q14155
- 途径
- EGFR Signaling Pathway, Neurotrophin Signaling Pathway, EGFR Downregulation
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