YWHAB Protein (AA 1-246) (Strep Tag)
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- 抗原 See all YWHAB 蛋白
- YWHAB (14-3-3 alpha + beta (YWHAB))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-246
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宿主
- 人
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资源
- Tobacco (Nicotiana tabacum)
- 标记
- This YWHAB protein is labelled with Strep Tag.
- 应用范围
- ELISA, Western Blotting (WB), SDS-PAGE (SDS)
- 序列
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MTMDKSELVQ KAKLAEQAER YDDMAAAMKA VTEQGHELSN EERNLLSVAY KNVVGARRSS WRVISSIEQK TERNEKKQQM GKEYREKIEA ELQDICNDVL ELLDKYLIPN ATQPESKVFY LKMKGDYFRY LSEVASGDNK QTTVSNSQQA YQEAFEISKK EMQPTHPIRL GLALNFSVFY YEILNSPEKA CSLAKTAFDE AIAELDTLNE ESYKDSTLIM QLLRDNLTLW TSENQGDEGD AGEGEN
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - 产品特性
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 内毒素水平
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product YWHAB 蛋白
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14-3-3 alpha + beta (YWHAB) (AA 1-246) protein (His tag)
YWHAB 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
14-3-3 alpha + beta (YWHAB) (AA 1-246) protein (GST tag)YWHAB 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
14-3-3 alpha + beta (YWHAB) (AA 2-246) protein (His tag)Crystallography grade YWHAB 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
14-3-3 alpha + beta (YWHAB) (AA 1-246) protein (Strep Tag)Crystallography grade YWHAB 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
14-3-3 alpha + beta (YWHAB) (AA 2-246) protein (His tag)Crystallography grade YWHAB 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
14-3-3 alpha + beta (YWHAB) (AA 1-246), (full length) protein (GST tag)YWHAB 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 95 % ELISA
14-3-3 alpha + beta (YWHAB) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)YWHAB 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
14-3-3 alpha + beta (YWHAB) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)YWHAB 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- YWHAB (14-3-3 alpha + beta (YWHAB))
- 别名
- YWHAB (YWHAB 产品)
- 别名
- GW128 Protein, HS1 Protein, KCIP-1 Protein, YWHAA Protein, 1300003C17Rik Protein, wu:fb22g04 Protein, wu:fr86a12 Protein, ywhab Protein, ywhab2 Protein, zgc:64092 Protein, 14-3-3beta Protein, fb80c08 Protein, fk41f08 Protein, wu:fb80c08 Protein, wu:fd11d01 Protein, wu:fk41f08 Protein, ywhab1 Protein, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta Protein, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide Protein, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide b Protein, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta Protein, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta S homeolog Protein, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta L homeolog Protein, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide a Protein, YWHAB Protein, Ywhab Protein, ywhabb Protein, ywhab.S Protein, ywhab.L Protein, ywhaba Protein
- 背景
- 14-3-3 protein beta/alpha (Protein 1054) (Protein kinase C inhibitor protein 1) (KCIP-1) [Cleaved into: 14-3-3 protein beta/alpha, N-terminally processed],FUNCTION: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2. Negative regulator of signaling cascades that mediate activation of MAP kinases via AKAP13. {ECO:0000269|PubMed:17717073, ECO:0000269|PubMed:19592491, ECO:0000269|PubMed:21224381}.
- 分子量
- 28.1 kDa
- UniProt
- P31946
- 途径
- Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Myometrial Relaxation and Contraction, Maintenance of Protein Location
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