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PROL1 Protein (AA 22-248) (His tag)

Crystallography grade PROL1 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
产品编号 ABIN3086623
发货至: 中国
  • 抗原 See all PROL1 蛋白
    PROL1 (Proline Rich, Lacrimal 1 (PROL1))
    蛋白类型
    Recombinant
    产品特性
    AA 22-248
    宿主
    • 2
    • 1
    • 1
    资源
    • 3
    • 1
    Insect Cells
    标记
    This PROL1 protein is labelled with His tag.
    应用范围
    ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)
    序列
    MQRFSRRPYL PGQLPPPPLY RPRWVPPSPP PPYDSRLNSP LSLPFVPGRV PPSSFSRFSQ AVILSQLFPL ESIRQPRLFP GYPNLHFPLR PYYVGPIRIL KPPFPPIPFF LAIYLPISNP EPQINITTAD TTITTNPPTT ATATTSTSTK PTMTISSSTV PISSTPEPAT SISAATPAAS TENTTQILAN RPHTVLLNAT VQVTTSNQTI LSSPAFKSFW QKLFAIFG HHHHHH
    Sequence including C-terminal His-tag.
    产品特性
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human PROL1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).
    • Tag Location: C-terminal His Tag

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    纯化方法
    The protein is purified from the cleared cell lysate using EDTA resistant His-tag capture materials. Eluate fractions are analyzed by SDS-PAGE. Protein containing fractions are subjected to a second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    纯度
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    过滤
    0.22 μm filtered
    内毒素水平
    Protein is endotoxin-free.
    质量等级
    Crystallography grade
  • 应用备注
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    说明

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    注意事项
    Avoid repeated freeze-thaw cycles.
    储存条件
    -80 °C
    储存方法
    Store at -80°C.
    有效期
    Unlimited (if stored properly)
  • 抗原
    PROL1 (Proline Rich, Lacrimal 1 (PROL1))
    别名
    PROL1 (PROL1 产品)
    别名
    BPLP Protein, PRL1 Protein, opiorphin Protein, Muc10 Protein, opiorphin prepropeptide Protein, proline rich, lacrimal 1 Protein, OPRPN Protein, Prol1 Protein
    背景
    Opiorphin is an endogenous inhibitor of neprilysin and aminopeptidase N. Inhibits the breakdown of substance P, Mca-BK2 and Met-enkephalin by neprilysin in vitro with IC(50) values of 29 uM, 33 uM and 33 uM respectively. Inhibits the breakdown of Ala-pNA by aminopeptidase N in vitro with an IC(50) of 65 uM. Has a potent analgesic effect when administered to rats by intravenous injection. {ECO:0000269|PubMed:17101991}.
    分子量
    24.9 kD. Including tag.
    UniProt
    Q99935
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